中国畜牧兽医2017，Vol.44Issue(1)：201-207,7.DOI:10.16431/j.cnki.1671-7236.2017.01.028
绵羊痘病毒固原株L2R基因的克隆与生物信息学分析
Cloning and Bioinformatics Analysis of L2R Gene of Sheeppox Virus GY Strain
摘要
Abstract
To explore the molecular characteristics of protein L2R from sheeppox virus(SPPV), genomic DNA was extracted from SPPV GY strain.The specific primers were designed and used to amplify the L2R gene from the genomic DNA by PCR.Then the PCR product was ligated into pGEM-T Easy vector.After transformation into E .coli Trans 5α,the positive clones were se-quenced and the sequences were analyzed by the bioinformatic softwares.The result showed that L2R gene sequence contained an open reading frame (ORF)of 279 nucleotides and deduced pro-tein consisted of 92 amino acids with the theoretical molecular weight of 10.92 ku and isoelectric point was 6.56.Analysis of secondary structure of protein L2R revealed that α-helix,β-strand, random coil and extended strand were 69.57%,9.78%,8.70% and 11.96%,respectively.Analy-sis of multiple sequence alignment showed that L2R gene from different capripox virus isolates were highly conserved,phylogenetic analysis showed that GY and NK,TU and SA was in a branch,indicating with a close genetic relationship among them.The present results laid a foun-dation for further studies of biological functions of protein L2R and interaction among the early proteins of capripox virus.关键词
羊痘病毒/L2R基因/基因克隆/生物信息学Key words
capripox virus/L2R gene/gene cloning/bioinformatics分类
农业科技引用本文复制引用
李杨,颜新敏,吴国华,李健,叶奕优,赵志荀,朱海霞,张强..绵羊痘病毒固原株L2R基因的克隆与生物信息学分析[J].中国畜牧兽医,2017,44(1):201-207,7.基金项目
国家重点研发计划课题（2016YFD0500907）；甘肃省国际科技合作专项（1604WKCA012）；甘肃省国际科技合作专项 （）
