中国畜牧兽医2017，Vol.44Issue(1)：227-235,9.DOI:10.16431/j.cnki.1671-7236.2017.01.032
上海地区屠宰场猪红斑丹毒丝菌的分离鉴定
Isolation and Identification of Erysipelothrix rhusiopathiae from Abattoirs in Shanghai Area
摘要
Abstract
In order to comprehend the status of Erysipelothrix rhusiopathiae carried in swinery in Shanghai area,samples of pigs were collected from some standard abattoirs in Shanghai area, including nasal swab,lung,tonsil and mandibular lymph nodes.Strains were isolated from these samples for detecting the Erysipelothrix rhusiopathiae .Several kinds of methods were used, such as VITEK 2 Compact system and VITEK MS system.A pair of primers was designed to amplify the 16S rRNA gene.16 PCR products selected randomly were sequenced and 16S rRNA homology analysis was conducted.As a result,strains were isolated from 49.07% (105/214) samples and were identified as Erysipelothrix rhusiopathiae .16S rRNA homology in strains of isolation and reference were 99.6% to 100.0%.The result indicated that the situation was very severe for so many carriers of Erysipelothrix rhusiopathiae in health group in nursery.关键词
屠宰场/猪红斑丹毒丝菌/分离鉴定Key words
abattoirs/Erysipelothrix rhusiopathiae/isolation and identification分类
农业科技引用本文复制引用
徐锋,王晓旭,张维谊,宁昆,齐新永,沈莉萍..上海地区屠宰场猪红斑丹毒丝菌的分离鉴定[J].中国畜牧兽医,2017,44(1):227-235,9.基金项目
《上海地区猪红斑丹毒丝菌的分离鉴定及毒力基因的检测》［沪农青字（2015）第2-7号；《上海地区主要动物病原微生物种质资源保存利用》［沪农科攻字］（2014）第7-3-3号］ （）
