中国畜牧兽医2017，Vol.44Issue(1)：236-241,6.DOI:10.16431/j.cnki.1671-7236.2017.01.033
蓝舌病1型病毒VP7蛋白表（拟）位定位及免疫检测分析
Epitope Mapping and Immuno-detection of VP7 Protein of Bluetongue Virus Serotype 1
摘要
Abstract
In this study,in order to map the key amino epitope of VP7 protein of blue tongue virus serotype 1 (BTV1),we used monoclonal antibody against VP7 protein of BTV1 to screen 7 mer phage display random peptide library.The selected phages including VP7 protein consensus se-quence were amplified and purified,immunoreactivity between epitope of selected phages and monoclonal antibody against VP7 of BTV1 was analyzed by ELISA and competitive ELISA (c-ELISA).The result showed that the epitope of phages including LNWPMVR sequence could spe-cially combine monoclonal antibody against VP7 of BTV1,and the combination could be inhibited or blocked by BTV1,it demonstrated that 7 mer phage simulated the antigenic determinant of BTV protein which could combine monoclonal antibody against VP7 of BTV1.The results sug-gested that the amino acides 163 to 189 (LNAGARGDVQQIFQGRNDPMMLYLVWR)were the specific epitope of VP7 protein of BTV.关键词
蓝舌病病毒/VP7蛋白/表位Key words
bluetongue virus/VP7 protein/epitope分类
农业科技引用本文复制引用
熊和丽,李国华,王金萍,苗海生,宋建领,李华春..蓝舌病1型病毒VP7蛋白表（拟）位定位及免疫检测分析[J].中国畜牧兽医,2017,44(1):236-241,6.基金项目
国家自然科学基金（31260612）；公益性行业（农业）科研专项 （）
