中国畜牧兽医2017，Vol.44Issue(1)：262-267,6.DOI:10.16431/j.cnki.1671-7236.2017.01.037
产肠毒素大肠杆菌K88诱导仔猪炎症反应的分子机制
Molecular Mechanism of the Inflammatory Response Induced by Enterotrxigenic E .coli K88 in Piglets
摘要
Abstract
To investigate the molecular mechanism of the inflammatory response in the piglets in-fected with enterotoxigenic E .coli (ETEC)K88,piglets were infected with ETEC K88,the IL-8 content in serum of piglets were assayed by ELISA,and the mRNA relative expression levels of TLR2/4 and its signal transduction pathway related genes (MyD88,Tollip and Bcl3)in mesenter-ic lymph nodes were detected by quantitative Real-time PCR.The results showed that compared with control group,the content of IL-8 in serum and the expressions of TLR2/4 in lymph nodes were all extremely significantly or significantly increased at 6 and 24 h after infection (P <0.01;P <0.05),and the IL-8 content and TLR2/4 mRNA expression at 24 h after infection were all significantly lower than those at 6 h after infection (P <0.05 ).In addition,the expressions of MyD88,Tollip and Bcl3 in lymph nodes were all extremely significantly increased at 24 h after in-fection compared with control group (P <0.01),but there was no significant difference between experimental group and control group at 6 h after infection (P >0.05).In conclusion,ETEC K88 infected piglets might produce inflammatory cytokines IL-8 through the TLR2/4-MyD88 signaling pathway,which could promote the inflammatory reaction in piglets.This inflammatory response might be regulated by Tollip and Bcl3,which could weak the inflammatory intensity in piglets.关键词
大肠杆菌K88/仔猪/炎症反应/Toll-样受体/炎症调控Key words
E .coli K88/piglet/inflammatory response/Toll-like receptors/inflammatory regula-tion分类
农业科技引用本文复制引用
李海花,朱琪,王世琼,杨春蕾,赵向华,乔家运,王文杰..产肠毒素大肠杆菌K88诱导仔猪炎症反应的分子机制[J].中国畜牧兽医,2017,44(1):262-267,6.基金项目
国家自然科学基金 （）
