中国畜牧兽医2017，Vol.44Issue(1)：275-281,7.DOI:10.16431/j.cnki.1671-7236.2017.01.039
复方忍冬藤提取物促进骨折愈合及抗炎作用研究
Effects of Mixed Caulis Lonicerae japonicae Extracts on Fracture Healing and Anti-inflammation
摘要
Abstract
This experiment was aimed to study the effects of mixed Caulis Lonicerae japonicae ex-tracts on fracture healing and inflammation.Rabbit right radius fracture model was induced by dental drill in order to study the medicine’s effect on fracture healing.Moreover,the contents of Ca,P and alkaline phosphatase in serum were measured to explore the influences of mixed Caulis Lonicerae japonicae extracts on serum biochemical indexes of fractured rabbits.RAW264.7 cell was induced by LPS in order to research the effect of medicine on inflammation.The concentra-tions of TNF-α,IL-1β,IL-6 and NO were measured by ELISA.The results showed that the rabbit fracture model was successfully prepared by dental drill,and the extracts of Caulis Lonicerae ja-ponicae could increase the levels of Ca and alkaline phosphatase and reduce the P content in the serum of fractured rabbits.The TNF-α,IL-1βand NO levels in RAW264.7 cell induced by LPS were significantly inhibited by the extracts with concentrations ranging from 50 to 200 μg/mL (P <0.05).However,the extracts had no significant effect on the level of IL-6 (P >0.05 ). These results demonstrated that the extracts of mixed Caulis Lonicerae japonicae could promote the fracture healing and possessed good resistance inflammation effect.关键词
复方忍冬藤/提取物/骨折愈合/抗炎Key words
mixed Caulis Lonicerae japonicae/extracts/fracture healing/anti-inflammation分类
农业科技引用本文复制引用
贾海燕,滕旭东,王慧,张慧珍..复方忍冬藤提取物促进骨折愈合及抗炎作用研究[J].中国畜牧兽医,2017,44(1):275-281,7.基金项目
国家级大学生创新创业训练计划项目（201510452025）；山东省科技发展计划项目 （）
