中国药房2017，Vol.28Issue(4)：465-468,4.DOI:10.6039/j.issn.1001-0408.2017.04.09
单针藻的化学成分及其体外抗菌、抗氧化活性研究
Study on Chemical Compounds and in vitro Antibacterial and Antioxidant Activities of Monoraphidium dy-bowskii
摘要
Abstract
OBJECTIVE:To study chemical compounds of Monoraphidium dybowskii,and to investigate the in vitro antibacte-rial and antioxidant activities of isolated compounds. METHODS:The ethanol extract of M. dybowskii were extracted with aether petrolei,ethyl acetate and n-butyl alcohol. The ethyl acetate extract was separated from M. dybowskii and chemical components were analyzed by sillica gel column chromatogram,HPLC and GC-MS. Their structures were identified according to physicochemi-cal properties and NMR. MIC of 4 isolated compounds to Pseudomonas aeruginosa,Candida albicans,Bacillus subtilis and Esche-richia coli were determined by resazurin disc test. Free radical scavenging rate(concluated by IC50)and reducing capacity were mea-sured by 1,1-diphenyl-2-picryl-diazanyl (DPPH) and ferric reducing antioxidant power (FRAP) assay. RESULTS:Compounds 1-6 were obtained from E4 and E5 segments of ethyl acetate extract of M. dybowskii,and their structures were identified as stigmas-terol,diisonoyladipate,indole-3-carboxylic acid,(+)-epiloliolide,(-)-loliolide,5-hydroxy-3,4-dimethy-5-pentyl-2(5H)-furanone. MIC of compounds 3-6 were 10-500 µg/mL,and IC50 ranged 22.02-71.01 µg/mL;FRAP ranged (62.04 ± 5.36)-(281.22 ± 8.3) µmol/L. CONCLUSIONS:M. dybowskii contains multiple lipid and alkanoic acid,and possesses certain in vitro antibacterial and antioxidant activities.关键词
单针藻/乙酸乙酯部位/萃取物/化学成分/鉴定/抗菌/抗氧化Key words
Monoraphidium dybowskii/Ethyl acetate/Extract/Chemical components/Identification/Antibacterial/Antioxi-dant分类
医药卫生引用本文复制引用
赵震宇,罗宁,陈晨,李昂,马莎莎,刘积光,王孟,刘平怀..单针藻的化学成分及其体外抗菌、抗氧化活性研究[J].中国药房,2017,28(4):465-468,4.基金项目
国家科技支撑计划项目（No.2011BAD14B01）；国家科技型中小企业技术创新基金项目（No.13C26244604892）；海南省中药现代化科技专项（No.ZY201327）；海南省产学研一体化项目 （）
