中国药房2017，Vol.28Issue(4)：472-475,4.DOI:10.6039/j.issn.1001-0408.2017.04.11
人参皂苷Rg1配伍乌头碱对体外培养心衰模型心肌细胞的保护作用
Protective Effect of Compatibilities of Ginsenosides Rg1 and Aconitine on Myocardial Cells of in vitro Cul-tured Heart Failure Model
摘要
Abstract
OBJECTIVE:To investigate the protective effect of the compatibilities of ginsenosides Rg1 and aconitine on myocar-dial cell of in vitro cultured heart failure model. METHODS:The myocardial cells of neonate rat were grouped into normal control group,model group,positive control group(Deslanoside injection,1×10-7 mol/L),ginsenosides Rg1 group(1×10-8 mol/L),acon-itine group (1 × 10-9 mol/L) or their compatibilities groups (1∶1,2∶1,1∶2,V/V). Except for normal control group,other groups were given 0.8%pentobarbital sodium to induce heart failure model of myocardial cells. After modeling,each group was given rele-vant medicine for 1 h,and then the activities of T-ATPase,Ca2+-Mg2+-ATPase,Na+-K+-ATPase in cells were all detected. The activi-ties of acyl carrier protein(ACP)and lactate dehydrogenase(LDH),and the contents of brain natriuretic party(BNP),TNF-α and total glycogen were measured in cell culture fluid. RESULTS:Compared with normal control group, T-ATPase and Ca2+-Mg2+-ATPase activities were decreased significantly in model group;meanwhile,Na+-K+-ATPase activity was increased signifi-cantly,and ACP,LDH activities and BNP content in cell culture fluid were increased significantly(P<0.05). Compared with mod-el group,the activities of T-ATPase in treatment groups were increased significantly,while the activity of LDH in cell culture fluid was decreased significantly;when the volume ratio of ginsenoside Rg1 to aconitine was 2∶1,protective effect was the strongest;the activities of Na+-K+-ATPase in aconitine group and compatibility groups were all decreased significantly,with statistical signifi-cance(P<0.05). The activities of ACP and BNP in cell culture fluid were all decreased in treatment groups,but the content of to-tal glycogen in cells and the TNF-α content of in cell culture fluid had no change (P>0.05). CONCLUSIONS:Compatibility of ginsenosides Rg1 and aconitine can improve ATPase activities and membranous permeability,regulate BNP secretion and protect myocardial cell of heart failure model,especially the compatibility of ginsenosides Rg1 to aconitine of 2∶1 ratio.关键词
人参皂苷Rg1/乌头碱/配伍/心衰模型/心肌细胞/细胞膜通透性/离子转运相关酶Key words
Ginsenosides Rg1/Aconitine/Compatibility/Heart failure model/Myocardial cell/Membranous permeability/Ion-related ATPase分类
医药卫生引用本文复制引用
董艳红,谢晓芳,李雪梅,朱雅宁,彭成..人参皂苷Rg1配伍乌头碱对体外培养心衰模型心肌细胞的保护作用[J].中国药房,2017,28(4):472-475,4.基金项目
国家自然科学基金重点项目（No.81630101）；国家科技支撑计划课题（No.2011BAI13B05）；“重大新药创制”科技重大专项（No.2013ZX09201018）；四川省2013年重点科技自筹项目（No.2013JYZ010，2013SZ0114）；四川省博士后科研项目 （）
