中国药房2017，Vol.28Issue(4)：479-482,4.DOI:10.6039/j.issn.1001-0408.2017.04.13
补肾活血化痰汤对多囊卵巢综合征模型大鼠卵巢血供及形态的影响
Effects of Bushen Huoxue Huatan Decoction on Ovarian Blood Supply and Form in Polycystic Ovarian Syn-drome Model Rats
摘要
Abstract
OBJECTIVE:To explore the effects of Bushen huoxue huatan decoction on ovarian blood supply and form in model rats with polycystic ovarian syndrome(PCOS). METHODS:50 SD female rats were randomized into normal control group,model group,positive control group [rosiglitazone,3 mg/(kg·d)],Bushen huoxue huatan decoction low-dose and high-dose groups [5.0, 7.5 g/(kg·d)],with 10 rats in each group. Except for normal control group,PCOS model was induced in the other groups by Poresky method. After modeling,treatment groups were given relevant medicine intragastrically. The normal control group and mod-el group were given normal saline [10 mL/(kg·d)] intragastrically,once a day,for 22 days. After the end of administration,the rats were sacrificed. The microvessel density (MVD) was recorded. mRNA expression of vascular endothelial growth factor (VEGF), ovarian angiotensin Ⅱ receptor(Ang Ⅱ)type 1(AT1)and type 2(AT2),G-protein coupled receptor MAS were all detected in ovarian tissue of rats. The ovarian form was observed. RESULTS:Compared with normal control group,MVD of mod-el group and treatment groups were all increased,while mRNA expression of AT1,AT2 and MAS were down-regulated (P<0.05);cystic sinus sollicle,the decrease of granular cell layer,egg mother cells disappearance,the decrease of luteal tissue and other obvious pathological changes could be found in ovarian tissue of rats in model group. Compared with model group,above in-dexes of treatment groups were improved significantly,and the effects of Bushen huoxue huatan decoction low-dose and high-dose groups were better than that of positive control group (P<0.05). The pathological changes of ovarian tissue in treatment groups were all improved to certain extent. CONCLUSIONS:Bushen huoxue huatan decoction can improve ovarian blood supply,angio-genesis and ovulation in PCOS rats. It is beneficial to improve ovarian form.关键词
补肾活血化痰汤/多囊卵巢综合征/卵巢形态/卵巢血供/大鼠Key words
Bushen huoxue huatan decoction/Polycystic ovarian syndrome/Ovarian form/Ovarian blood supply/Rats分类
医药卫生引用本文复制引用
王瑞杰,李晖,邱方,王自闯..补肾活血化痰汤对多囊卵巢综合征模型大鼠卵巢血供及形态的影响[J].中国药房,2017,28(4):479-482,4.基金项目
河南省教育厅科学技术研究重点项目（No.13A360579）；全国名老中医药专家传承工作室建设项目（No.〔2014〕-20号）；河南中医学院博士启动基金 （）
