中国药房2017，Vol.28Issue(4)：518-520,521,4.DOI:10.6039/j.issn.1001-0408.2017.04.24
蒙药山野豌豆多糖的提取与含量测定
Extraction and Content Determination of Polysaccharide in Mongolian Medicine Vicia amoena
李振 1云学英 1于海青 1李成林 1郭晓宇 1况媛媛1
作者信息
- 1. 内蒙古医科大学药学院，呼和浩特 010110
- 折叠
摘要
Abstract
OBJECTIVE:To optimize the decolorization condition for polysaccharide extract of Mongolian medicine Vicia amoena,and to establish the method for its extraction and content determination. METHODS:The water extract-alcohol precipita-tion was used to extract polysaccharide from Mongolian medicine V. amoena. Using decolorization rate as index,orthogonal test was designed to investigate the effects of the dosage of activated carbon,decolorization temperature,decolorization time on the de-colorization of polysaccharide,so as to optimize the conditions for the decoloration of polysaccharide. Using glucose as control, phenol sulfuric acid method was adopted,and the content of polysaccharide in crude polysaccharide was determined by UV spectro-photometry at 490 nm. RESULTS:The optimal decoloration condition was as follows as actived carbon of 3%,decoloration time of 40 min,decoloration temperature of 60 ℃. On this basis,the average decolorization rate reached 19.77%(RSD=1.85%,n=3) by the verification test of the decoloration. The average extraction yield for the crude polysaccharide was 4.56%(RSD=2.38%,n=3),of which the polysaccharide content was 1.98%(RSD=2.18%,n=4). CONCLUSIONS:This experiment has relatively good reproducibility in polysaccharide yield;established method for content determination of polysaccharide is stable and feasible.关键词
蒙药/山野豌豆/多糖/分光光度法/含量测定/活性炭/脱色率/正交试验Key words
Mongolian medicine/Vicia amoena/Polysaccharide/Spectrophotometry/Content determination/Activated car-bon/Decolorization rate/Orthogonal test分类
医药卫生引用本文复制引用
李振,云学英,于海青,李成林,郭晓宇,况媛媛..蒙药山野豌豆多糖的提取与含量测定[J].中国药房,2017,28(4):518-520,521,4.
