中国药房2017，Vol.28Issue(4)：533-535,536,4.DOI:10.6039/j.issn.1001-0408.2017.04.28
抗肿瘤药H6聚合物胶束的制备及体外抗肿瘤作用研究
Study on Preparation of Anti-tumor Drug H6 Polymeric Micelles and Their in vitro Anti-tumor Effects
潘超 1刘会丽 2许俊鹏 3唐俏欣 1万丽1
作者信息
- 1. 成都中医药大学药学院，成都 611137
- 2. 四川大学生物治疗国家重点实验室，成都 610041
- 3. 四川大学生物治疗国家重点实验室，成都 610041
- 折叠
摘要
Abstract
OBJECTIVE:To prepare anti-tumor drug H6(lactone compound)polymeric micelles,and to investigate its in vitro anti-tumor effects. METHODS:Using mPEG2000-PCL4000 as carrier,H6/mPEG2000-PCL4000 micelles were prepared. Using particle size, PDI and 48 h whether to produce precipitation as indexes,feeding ratio,H6 concentration,volume ratio of organic solvent were screened. The encapsulation efficiency and drug-loading amount of micelle were all detected. MTT assay was used to detect the tox-icity of micelles and H6 solution to human non-small cell lung cancer cell A549 and human lung cancer cell H460. RESULTS:The screened formulation was as follows as feeding ratio of 1∶25,H6 concentration of 2 mg/mL,the ratio of ethanol to chloroform of 1∶1(V/V). The parameters of prepared H6/mPEG2000-PCL4000 micelles were as follows as particle size of(40.74±0.116 3)nm,PDI of(0.101±0.006),encapsulation efficiency of(94.87±0.016 3)%,drug-loading amount of(7.07±0.001 5)%(n=3). IC50 of mi-celles and H6 solution to A549 cell were 15.62 and 12.57 nmol/L;IC50 of micelles and H6 solution to H460 cell were 27.68 and 15.19 nmol/L. CONCLUSIONS:H6/mPEG2000-PCL4000 micelles are prepared successfully and show in vitro anti-tumor effects.关键词
抗肿瘤药H6/mPEG2000-PCL4000/胶束/抗肿瘤作用Key words
Anti-tumor drug H6/mPEG2000-PCL4000/Micelles/Anti-tumor effect分类
医药卫生引用本文复制引用
潘超,刘会丽,许俊鹏,唐俏欣,万丽..抗肿瘤药H6聚合物胶束的制备及体外抗肿瘤作用研究[J].中国药房,2017,28(4):533-535,536,4.
