中国药理学通报2017，Vol.33Issue(1)：33-38,39,7.DOI:10.3969/j.issn.1001-1978.2017.01.007
过表达Nampt通过激活NF-κB诱导心肌细胞肥大
Overexpression of Nampt induced cardiac hypertrophy by activating NF-κB
摘要
Abstract
Aim To examine the effect of Nampt over-expression on cardiac hypertrophy,and elucidate the role of NF-κB.Methods The cultured neonatal car-diomyocytes were pretreated with 100 μmol · L-1 PE or transfected with Nampt.The mRNA and protein ex-pression of Nampt were determined by Real-time PCR and Western blot respectively.The cardiomyocyte hy-pertrophy was monitored by measuring cell-surface area and the mRNA levels of ANP and BNP,which were biomarkers of hypertrophic response.Moreover,we te-sted the effects of Nampt on NF-κB-dependent tran-scription activity through luciferase reporter gene as-says.Results Nampt overexpression significantly in-creased cardiomyocyte surface area and the mRNA ex-pression of ANP and BNP.In addition,Nampt overex-pression could markedly increase NF-κB-dependent transcription activity. Moreover, when p65 was knocked down,cardiomyocytes with Nampt overexpres-sion could not induce cardiac hypertrophy.Conclusion <br> Overexpression of Nampt induces cardiac hypertro-phy by increasing NF-κB-dependent transcription activ-ity.关键词
Nampt/心肌肥大/NF-κB/苯肾上腺素/ANP/BNPKey words
Nampt/cardiac hypertrophy/NF-κB/PE/ANP/BNP引用本文复制引用
蔡轶,黄珺珺,刘夏雯,黄碧云,朱柳,吴波..过表达Nampt通过激活NF-κB诱导心肌细胞肥大[J].中国药理学通报,2017,33(1):33-38,39,7.基金项目
国家自然科学基金资助项目（No 81300085）；广州市科技计划－科学研究专项（No 201510010179）；广东省科技计划项目－广东省高科技发展专项项目（No 2013B010403024）；广州市属高校科研计划项目 （）
