中国药理学通报2017，Vol.33Issue(1)：39-44,6.DOI:10.3969/j.issn.1001-1978.2017.01.008
山奈酚对慢性脑缺血大鼠学习记忆能力的影响及机制探讨
Effects of kaempferol on learning and memory in rats with chronic cerebral ischemia and possible mechanisms
摘要
Abstract
Aim To investigate the effects and possible mechanisms of kaemperol in the rats with chronic cere-bral ischemia.Methods Chronic cerebral hypoperfu-sion model was produced by permanent occlusion of bi-lateral common carotid arteries (2VO)in rats.After KAE treatment,the rats underwent Morris water maze and prehensile traction test.Neuronal morphology was observed using Nissl and HE staining.The activity of SOD and the content of MDA in brain tissue were de-termined.The DJ-1 protein expression was assayed by Western blot.Results Compared with 2VO model group,KAE significantly improved learning and memo-ry and the grasping ability.In addition,KAE signifi-cantly reduced brain tissue pathological injury induced by 2VO. Furthermore, KAE significantly increased SOD activity and enhanced antioxidant protein DJ-1 ex-pression in brain tissue.Conclusions KAE could sig-nificantly attenuate the cognitive impairment,limb bal-ance dysfunction and pathological injury in rats with chronic cerebral ischemia.The mechanism may be re-lated to improving the antioxidant system in vivo.关键词
山奈酚/血管性痴呆/慢性脑缺血/慢性脑低灌注/双侧颈总动脉永久结扎模型/学习记忆/超氧化物歧化酶/DJ-1 蛋白Key words
kaempferol/vascular dementia (VD )/chronic cerebral ischemia/chronic cerebral hypoperfu-sion(CCH)/bilateral common carotid arteries occlu-sion (2-VO )/learning and memory/superoxide dis-mutase(SOD)/DJ-1 protein引用本文复制引用
张君,程笑,杨欢,杨滢霖,赵廷坤,王奇,王月华,杜冠华..山奈酚对慢性脑缺血大鼠学习记忆能力的影响及机制探讨[J].中国药理学通报,2017,33(1):39-44,6.基金项目
国家自然科学基金面上项目（No 81473383，81573645）；国家科技部“重大新药创制”科技重大专项（No 2012ZX09103101-078）；中国医学科学院医学与健康科技创新工程 （）
