中国药理学通报2017，Vol.33Issue(1)：100-104,5.
鬼臼毒素衍生物LN-13诱导多药耐药细胞K562／A02凋亡及其机制
Podophyllotoxin derivative LN-13 induced multidrug resistant cell K562/A02 apoptosis and its molecular mechanism
摘要
Abstract
Aim To study the mechanism of action of the new derivative of podophyllotoxin(LN-13)in indu-cing the apoptosis of K562/A02 cells.Methods The MTT method was taken to detect the inhibition of LN-13 and VP-16 on K562/A02 proliferation and inhibi-tion rate and IC50 values were obtained 48 hours later. The K562/A02 cell morphological change induced by LN-13 were observed through Hochest33342 and PI staining after 48 hours later.Flow cytometry was taken to detect the apoptosis of K562/A02 cells induced by LN-13.The reverse transcription-polymerase chain re-action was taken to detect the Bcl-2,Bax,caspase-3 and mdr-1 mRNA expression.The expression of P-gp was detected by Western blot.Results The growth of K562 /A02 cells was obviously inhibited by LN-13 when IC50 value was 3.32 μmol · L-1 .LN-13 could obviously induced cell apoptosis observed by Ho-chest33342 and PI staining.Flow cytometry detection showed that LN-13(2,4,8 μmol·L-1 )could induce cell apoptosis and apoptosis ratio reached 15.0%, 48.0%,68.96%,respectively.The reverse transcrip-tion-polymerase chain reaction showed that LN-13 in-creased the Bax and Caspase-3 mRNA expression,and meanwhile the expression of mdr-1 mRNA decreased. Western blot showed that P-gp expression was de-creased as the LN-13 dose increased.The data were significantly different from those of control group.Con-clusion Podophyllotoxin derivative LN-13 can induce the apoptosis of K562 /A02 cells,which may be close-ly-related to regulating P-gp expression and apoptosis related gene mRNA expression.关键词
K562 细胞/K562/A02 细胞/新型鬼臼毒素衍生物LN-1 3/凋亡/P糖蛋白/多药耐药/凋亡相关基因Key words
K562/K562/A02 cells/podophyllotoxin derivatives LN-13/apoptosis/P- glycoprotein/multidrug resistance/apoptosis-related genes引用本文复制引用
高晨光,张猜,赵安妮,李楠,陈虹,曹波..鬼臼毒素衍生物LN-13诱导多药耐药细胞K562／A02凋亡及其机制[J].中国药理学通报,2017,33(1):100-104,5.基金项目
天津市应用基础与前沿技术研究计划青年项目（No 15JCQNJC13500）；武警后勤学院中心实验室开放基金资助项目 （）
