中国医学创新2016，Vol.13Issue(35)：24-26,27,4.DOI:10.3969/j.issn.1674-4985.2016.35.006
靶向沉默RACK1基因对肺腺癌裸鼠移植瘤生长的抑制作用
Inhibitory Effect of Targeted Silencing RACK1 Gene on the Growth of Lung Adenocarcinoma Xenograft in Nude Mice
摘要
Abstract
Objective:To study the effect of silencing RACK1 gene on the growth of human lung adenocarcinoma cell line A549 in nude mice.Method:BALB/C nude mice were subcutaneously transplanted with lung adenocarcinoma cell lines A549 and tumor bearing nude mice were randomly divided into 3 groups when the tumor diameter was 0.3-0.5 cm,and 6 rats in each group,they were injected with normal saline,empty plasmid liposome complex and shRNA plasmid liposome complex.The tumor volume changes of the three groups were observed,the tumor volume of nude mice were recorded and the expression of RACK1 protein was detected by Western blot.Result:28 days after tumor formation,there were obvious decreases in tumor growth rate, tumor mass,as well as tumor weight in transfected group,comparing with empty vector transfeeted group and untransfected group(P<0.05).Transfection of RNA interference can inhibit the growth of xenograft tumor by 39%. Western blot revealed that the expression of RACK1 protein of transfected group were significantly lower than those in untransfected group and empty vector transfected group(P<0.05).Conclusion: Silencing RACK1 gene can effectively inhibit the growth of lung adenocarcinoma in nude mice.关键词
肺腺癌/RACK1基因/裸鼠Key words
Lung adenocarcinoma/RACK1 gene/Nude mice引用本文复制引用
米小芳,高小云,梁钢,杜凯丽,王宏坤,肖虹,梁建芳,郑绘霞..靶向沉默RACK1基因对肺腺癌裸鼠移植瘤生长的抑制作用[J].中国医学创新,2016,13(35):24-26,27,4.基金项目
山西省科技攻关项目 （）
