中国医学创新2016，Vol.13Issue(35)：27-30,4.DOI:10.3969/j.issn.1674-4985.2016.35.007
LY294002对高糖培养原代足细胞snail表达的影响
Effect of LY294002 on Snail of Glomerular Podocytes under High Glucose Condition in Rats
摘要
Abstract
Objective:To evaluate the effect of the blocker of PI3K LY294002 on the expression of snail in high glucose-induced podocyte cell.Method:The glomeruli were isolated and gathered for primary culture. The cells were randomized into normal glucose(NG) group,high glucose(HG) group,mannitol control(MG) group,HG+LY294002(2 mg/L)group. The culture was continued for 48 h.The expression of snail was observed with Western blot methods.Result:Compared with NG group,the podocytic-process retraction and partial effacement and intercellular junction losing appeared in HG group,the expression of snail was increased(P<0.05). Compared with HG group,there was a down-regulation of the expression of snail(P<0.05).Conclusion:PI3K inhibitor protects podocytes by partly inhibiting the expression of snail.关键词
高糖/足细胞/PI3K抑制剂/snailKey words
Glucose/Podocytes/LY294002/Snail引用本文复制引用
贾苗,谢玉贤,邱宏,李冰燕..LY294002对高糖培养原代足细胞snail表达的影响[J].中国医学创新,2016,13(35):27-30,4.基金项目
苏州科技计划项目 （）
