中国医学创新2016，Vol.13Issue(36)：14-17,4.DOI:10.3969/j.issn.1674-4985.2016.36.004
抗胶质瘤Ⅰ号方对脑胶质瘤细胞侵袭性的影响
The Effect of Resistance to GliomaⅠPrescriptions on the Invasion of Glioma Cells
摘要
Abstract
Objective:To investigate the effect of Resistance to GliomaⅠPrescriptions on reducing the invasiveness of glioma cells,and the relationship between Resistance to GliomaⅠPrescriptions and PI3K/PKB signal transduction pathway.Method:MTT method was used to detect cell inhibition rate of C6 glioma cells which was treated by the Resistance to GliomaⅠPrescriptions,the expression level of PI3K and PKB in tumor cells was detected by Western blot,in the end,the number of glioma cells in Transwell was detected by crystal violet staining method.Result:Resistance to GliomaⅠPrescriptions can inhibit PI3K and PKB protein expression level, the expression level of PI3K protein was the lowest(25.1±3.1)when the effect of Resistance to GliomaⅠwas 0.5 h,and the lowest level of PKB protein expression was(25.1±3.8)at the effect of Resistance to gliomaⅠwas 1 h,compared with the control group(99.1±1.6),(91.2±3.5),the differences were statistically significant (P<0.05).While the largest inhibition rate appear at 3 h after Resistance to GliomaⅠPrescriptions,inhibition rate was(91.2±5.7)%,compared with(68.0±5.2)%in control group,the difference was statistically significant (P<0.01).At the same time,the glioma cell number on transwell chamber surface was the least(1.4±0.4) ×106,compared with(35.6±2.7)×106 in control group,the difference was statistically significant(P<0.05). Conclusion:Resistance to GliomaⅠPrescriptions may is through the inhibition of PI3K/PKB signal transduction pathway and inhibit the proliferation of glioma cells,thereby reducing the invasiveness of glioma cells.关键词
胶质瘤/抗胶质瘤Ⅰ号/PI3K/PKB信号传导通路Key words
Glioma/Resistance to GliomaⅠPrescriptions/PI3K/PKB signal transduction pathway引用本文复制引用
刘春玲,唐明科,秦丽娟,赵喜庆..抗胶质瘤Ⅰ号方对脑胶质瘤细胞侵袭性的影响[J].中国医学创新,2016,13(36):14-17,4.基金项目
国家自然科学基金项目（81101912）；河北省中医药管理局中医药类科研项目（2014195）；河北省高等学校科学技术研究项目 （）
