摘要
Abstract
Objective:IMMS is a new method of collecting body fluid tumor cells,and it is used to find out whether there is no shedding of tumor cells in the body fluid of the patients.Method:The tumor cells were collected and compared with the conventional sedimentation centrifugation method.Body fluids were collected from 85 patients with tumor and 53 patients with non tumor,in the same volume of body fluids,immune magnetic microsphere suspension and 138 patients with body fluids(urine sediment,ascites,peritoneal effusion,pleural effusion,nipple discharge)mixture,the collected suspicious cells in conventional microscopy,and separated the microscopic examination of the 138 patients with body fluids sediment precipitation centrifugal,compared the two kinds of mirror inspection results.Result:The positive rate of immune magnetic microsphere detection method in non tumor patients was 1.9%(1/53),the positive rate of precipitation centrifugation test method was 0(0/53).The positive rate of immune magnetic microsphere detection in patients with hepatocellular carcinoma was 91.6%(11/12),the positive rate of precipitation centrifugation test method was 33.3%(4/12).The positive rate of immune magnetic microsphere detection in patients with colon cancer was 88.9%(24/27),the positive rate of precipitation centrifugation test method was 37.0%(10/27).The positive rate of the detection method of the immune magnetic microsphere in patients with lung cancer was 80.0%(12/15),the positive rate of precipitation centrifugation test method was 40.0%(6/15).The positive rate of immune magnetic microsphere detection in patients with breast cancer was 90.3%(28/31),the positive rate of precipitation centrifugation test method was 45.2%(14/31).In patients with non tumor and tumor patients,the positive detection rate of the immune magnetic microsphere detection method were higher than those in precipitation centrifugation test method(P<0.05). Conclusion:Immune magnetic microsphere detection method easily ruled out normal cells mixed in body fluids in the sediment,broken cells and non cellular components,excluding factors interference microscopy,increase the screening concentration of positive cells in efforts to improve the positive detection rate,has a great effect on tumor diagnosis,pathological diagnosis,clinical tumor cytology more effective and convenient method.关键词
免疫磁微球/恶性肿瘤/体液中瘤细胞Key words
IMMS/Malignant tumor/Body fluids tumor cells
