中国医药科学2016，Vol.6Issue(21)：38-41,4.
紫檀芪通过PI3K-AKT信号途径抑制缺氧/复氧诱导的乳鼠心肌细胞凋亡
Pterocarpus on apoptosis of neonatal rat cardiomyocytes induced by hypoxia/reoxygenation through the PI3K-AKT signal pathway
摘要
Abstract
Objective To investigate the effect of Pterocarpus on apoptosis of neonatal rat cardiomyocytes induced by hypoxia / reoxygenation and the effect of phosphatidylinositol 3-kinase (PI3K) / protein kinase B (AKT) signaling pathway. Methods Cardiomyocytes of neonatal rats were subjected to hypoxia/reoxygenation (H/R), and the hearts were subjected to myocardial ischemia-reperfusion injury. The rats were divided into normal group, model group (H/ R) and pterocarpus stilbene group (H/R+0.5,5,50μmol/L).Cell viability was measured by MTT assay. Cardiomyocyte morphology was detected by inverted microscope. Annexin V-FITC/PI flow cytometry was used to detect the apoptosis of cardiomyocytes.PI3K/AKT activation was analyzed by Western blot. Results Compared with the normal group, the center of model group of muscle cell was shrinkage, rounding and brightening, and the cell viability decreased, early and late apoptosis rate of cells increased significantly. The expression of PI3K and the phosphorylation of AKT in the model group were significantly higher than those in the model group (P < 0.05). Compared with the model group, 5, 50mol/L of pterostilbene group center muscle morphological was recovery, cell viability was increased, and the rosewood the expression of PI3K was increased, and the difference was significant (P<0.05). 0.5,5,50 mol/L pterostilbene group the apoptosis of myocardial cells decreased, the phosphorylation level of AKT increased, the difference was significant (P < 0.05). Conclusion Pterocarpus can resist cardiomyocyte apoptosis induced by hypoxia / reoxygenation, and activation of PI3K/AKT signal is the main way of its expression.关键词
紫檀芪/乳鼠心肌细胞/缺氧/复氧/细胞凋亡Key words
Pterocarpus stilbene/Neonatal rat cardiomyocytes/Hypoxia/reoxygenation/Cell apoptosis分类
医药卫生引用本文复制引用
刘峰,董少红,吴美善,梁瑞娟,熊玮,刘华东,陈科奇,林峰..紫檀芪通过PI3K-AKT信号途径抑制缺氧/复氧诱导的乳鼠心肌细胞凋亡[J].中国医药科学,2016,6(21):38-41,4.基金项目
广东省深圳市博士创新项目（201605004）。 （）
