中国医药科学2016，Vol.6Issue(21)：56-59,4.
毒热清颗粒提取工艺优选
Optimization of extraction technology for dureqing granule
摘要
Abstract
Objective To optimize the extraction process of dureqing granule. Methods Baicalin and chlorogenic acid were used as indexes, HPLC was used to determined content. Agilent ZORBAX XDB-C18 colume (4.6mm×150mm,5μm), Mobile phase methanol-0.4%phosphoric acid gradient elution with flow rate of 1.0 mL/min, UV detection wavelength was 280 nm(baicalin)and 327nm(chlorogenic acid ). Amount of water added, extraction time, and extraction times were selected as the factors. L9(34)orthogonal test was designed to optimize the extraction technology of Dureqing Keli. Results Optimal water extraction technology was as follows:adding 12 times amount of water, reflux and extract for 2 h, three times totally. Conclusion The optimized technology was simple and extract completely, it is applied to industrial manufacture.关键词
毒热清颗粒/黄芩苷/正交试验/高效液相色谱法/提取工艺Key words
Dureqing granule/Bbaicalin/Chlorogenic acid/Orthogonal design/HPLC/Extraction process分类
医药卫生引用本文复制引用
施钧瀚,秦会珍,郑弘,孟祥乐..毒热清颗粒提取工艺优选[J].中国医药科学,2016,6(21):56-59,4.基金项目
国家自然科学基金·河南省人民政府人才培养联合基金（ U1304824）；中国博士后科学基金特别资助（2015T80772）；中国博士后科学基金面上资助（2015M582190）；河南省博士后基金项目（2014-75）；济源市科技攻关项目(15013034)；河南省科技厅“三区”人才计划（14104259）。 （15013034）
