中国医药科学2016，Vol.6Issue(21)：67-70,4.
替加环素微生物限度方法学验证研究
Research of microbial limit methodology validation of tigecycline
摘要
Abstract
Objective To establish a method for microbial limit test on tigecycline. Methods The sample (5g) was dispersed uniformly to the nutrient broth medium (100mL),which was centrifuged for 3 minutes at 500r/min.The supernatant was diluted with the ratio of 1:20 as the test solution,and the filter membranes were incubated with the test solution (1mL) respectively,which was rinsed by the sterile solution(600mL) containing 0.3%tween-80 and 0.1%peptone,with the aim of eliminating the interference of tigecycline's bacteriostatic activity,to verify the method of Tigecycline's microbial limit test. Results With the method above,the recoveries of all the five validated bacteria were over 70%. Conclusion The membrane filtration method,which is that the membrane-filter is rinsed by the sterile solution (600mL) containing 0.3% tween-80 and 0.1% peptone,and it can be applied for the test of Tigecycline’s microbial limit.关键词
替加环素/微生物限度/方法学验证/吐温80Key words
Tigecycline/Microbial limit test/Methodology validation/Tween-80分类
医药卫生引用本文复制引用
陈伟娜,王燕清,陈嘉璐..替加环素微生物限度方法学验证研究[J].中国医药科学,2016,6(21):67-70,4.基金项目
广东省珠海市高新技术领域科技攻关及高新技术产业化项目（2012D0201990040）。 （）
