中国医药科学2016，Vol.6Issue(22)：22-25,4.
1MTA2基因沉默对人乳腺癌MCF-7/ADR多药耐药的逆转作用
The reversal effect of MAT2 silencing on multidrug-resistant breast cancer cell MCF-7/ADR
摘要
Abstract
Objective Breast cancer is the most common malignant tumor in women.To investigate the effect and mechanism of MTA2 on breast cancer drug resistance. Methods CCK-8 was used to detect the drug resistance of MCF-7/ADR and MCF-7 cells to adriamycin.MTA2 sequence specific shRNA was synthesized in vitro and transfected into human breast cancer cell line by lentivirus.Knockdown efficiency of lentivirus mediated MTA2 and the signaling pathway protein,such as PI3K,AKT and NF-κB,were detected by Western blot.Apoptosis was detected by Annexin V-FITC and DAPI staining. Results MTA2 expression in MCF-7/ADR cells was significantly higher than that in MCF-7 cell line.Lentivirus-mediated gene silencing significant knockdown MTA2 in MCF-7/ADR cells.Knockdown of MTA2 inhibited the activation of the downstream NF- kappa B pathway by inhibiting the PI3K or AKT pathway,and reversed the resistance to P-gp protein expression. Conclusion Knockout MTA2 can reverse the MCF-7/ADR cells drug resistant effect and these suggest MTA2 may be involved in regulating the role of breast cancer multidrug resistance.关键词
MTA2/乳腺癌/耐药Key words
MTA2/Breast cancer/Drug resistant分类
医药卫生引用本文复制引用
严梅娣..1MTA2基因沉默对人乳腺癌MCF-7/ADR多药耐药的逆转作用[J].中国医药科学,2016,6(22):22-25,4.基金项目
浙江省宁波市医学科技计划项目（2014A39）。 （）
