浙江农林大学学报2017，Vol.34Issue(1)：14-19,6.DOI:10.11833/j.issn.2095-0756.2017.01.003
雷竹对不同形态氮素养分的生理响应
Antioxidant system response to different forms and ratios of nitrogen in leaves and roots of Phyllostachys violascens
摘要
Abstract
Nitrogen (N), a necessary plant nutrient for plant growth can be applied as nitrate nitrogen (NO3--N) or ammonium nitrogen (NH4+-N) but may have different effects on nutrient absorption and physiological metabolism of the plant. To determine a proper N fertilizer for Phyllostachys violascens cultivation, an experi-ment supplying N fertilizer with five different ratios of NO3--N and NH4+-N (1∶0, 2∶1, 1∶1, 1∶2, and 0∶1) was conducted. Contents of malondialdehvde (MDA), soluble protein, and antioxidant enzyme activities were determined. Results showed that for the same treatment ratios of NO3--N and NH4+-N, MDA content, soluble protein, and antioxidant enzyme activities were higher in leaves than in roots. As the concentration of NH4+-N increased, the MDA content in leaves and roots first decreased and then increased with the lowest MDA having a ratio of 1 ∶ 1. Antioxidant enzyme activities first increased and then generally decreased having higher levels with mixotrophism. Superoxide dismutase (SOD) and catalase (CAT) activities in leaves were highest with the 1 ∶ 2 ratio; whereas SOD activity in roots was highest with the 1 ∶ 1 ratio, and CAT and peroxidase (POD) ac-tivities were highest with the 1 ∶ 2 ratio. Soluble protein content also increased first and then decreased with the highest for leaves in a 1 ∶ 2 ratio and for roots in a 1 ∶ 1 ratio. With mixed nutrition, MDA content was lower, but soluble protein content and antioxidant enzyme activities remained at much higher levels. Thus, with a mixture of NO3--N and NH4+-N at a 1 ∶ 1 ratio, the degree of oxidative damage to leaves and roots of Phyl-lostachys violascens was least, and antioxidant enzyme activities were stronger, thereby boosting growth and biomass accumulation.关键词
植物生理学/雷竹/铵态氮/硝态氮/硝铵比/抗氧化系统Key words
plant physiology/Phyllostachys violascens/nitrate nitrogen/ammonium nitrogen/nitrate/ammonium ratio/antioxidant system分类
农业科技引用本文复制引用
叶莉莎,陈双林..雷竹对不同形态氮素养分的生理响应[J].浙江农林大学学报,2017,34(1):14-19,6.基金项目
浙江省-中国林业科学研究院省院合作项目（2013SY12）；中央级公益性科研院所基本科研业务费专项资金项目 （）
