浙江农林大学学报2017，Vol.34Issue(1)：137-144,8.DOI:10.11833/j.issn.2095-0756.2017.01.019
3个白桦BpBEE基因的克隆与表达分析
Cloning and expression of three BpBEE genes in Betula platyphylla
摘要
Abstract
Based on the transcriptome sequences from 45 libraries of stem tips, leaves, and xylem of Betula platyphylla (birch), three BEE genes (BpBEE1, BpBEE2, and BpBEE3), were obtained bioinformatics and expression patterns of three genes were analyses. Results showed that three BpBEE genes belonged to basic helix-loop-helix (bHLH) transcription factor superfamily, and contained a typical DNA binding domain and E-box/N-box specificity sites. These genes were highly homologous to AtBEE genes, and belonged to the 25th sub-class in the bHLH superfamily. During the growing season, the RNA level of all three BpBEE genes were high-estin leaves than in others tissues. In the vigorous growing stage, All BpBEE genes were up-regulated in leaves and buds. With the brassinosteroids (BR) treatment, BpBEE1 was up-regulated in buds and xylem (> 2 times) in the early stages (2 h and 4 h); whereas, BpBEE2 was up-regulated in the xylem and buds in the early stage (2 h). These results suggested that the three BpBEE genes could be involved in the growth process of birch, and response for BR hormone, BpBEE1 and BpBEE2 showeds early reply responses, especially.关键词
林木育种学/白桦/bHLH转录因子/基因克隆/基因表达分析Key words
forest tree breeding/Betula platyphylla/bHLH transcription factors/gene cloning/gene expression analysis分类
农业科技引用本文复制引用
武丹阳,杨洋,李慧玉..3个白桦BpBEE基因的克隆与表达分析[J].浙江农林大学学报,2017,34(1):137-144,8.基金项目
“十二五”国家科技计划农村领域项目 （）
