北京林业大学学报2017，Vol.39Issue(1)：78-84,7.DOI:10.13332/j.1000-1522.20160179
舞毒蛾小分子热激蛋白基因分析及对甲萘威胁迫的响应
Analysis of smHsp genes in Lymantria dispar and its response to carbaryl stress
摘要
Abstract
Small heat shock proteins of insect with molecular weight from 12 to 43 kDa are stress proteins with molecular chaperone activity and can assist protein folding and transporting under environmental stress. In present study, the full length of six smHsp cDNA was identified from Lymantria dispar transcriptome. The open reading frames ( ORF) of smHsps were from 444 to 567 bp encoding proteins of 147--188 amino acid residues with the theoretical pI of 5. 58--6. 17 . These smHsps were named as LdHsp17. 0 , LdHsp18. 7 , LdHsp19. 1, LdHsp20. 3, LdHsp21. 3 and LdHsp21. 4 according to their molecular mass. The effects of sublethal concentrations ( LC5 , LC10 and LC30 ) of carbaryl on smHsp gene expression in L. dispar larvae were analyzed using real-time RT-PCR technology. The results showed that the expressions of LdHsp20. 3 , LdHsp19. 1 and LdHsp17. 0 were induced while those of LdHsp21. 4 , LdHsp21. 3 and LdHsp18. 7 were inhibited under three sublethal concentrations of carbaryl. The smHsp gene expressions were in concentration- and time-dependent way. Our study provides the basis for further studying the functions of smHsp and their roles in insecticide resistance in L. dispar.关键词
舞毒蛾/smHsp/甲萘威/表达分析Key words
Lymantria dispar/smHsp/carbaryl/expression analysis分类
农业科技引用本文复制引用
刘鹏,孙丽丽,张琪慧,曹传旺..舞毒蛾小分子热激蛋白基因分析及对甲萘威胁迫的响应[J].北京林业大学学报,2017,39(1):78-84,7.基金项目
国家自然科学基金项目(31570642、31101676)、黑龙江省自然科学基金面上项目(C201409)。 （31570642、31101676）
