草业学报2017，Vol.26Issue(1)：23-33,11.DOI:10.11686/cyxb2016196
高寒区施肥和豆科混播水平对燕麦人工草地土壤酶活性的影响
Soil enzyme activities in alpine naked oat-artificial grassland in response to fertilizer and legume mix levels
摘要
Abstract
To investigate the dynamics of soil enzymatic activity with regard to the combined effects of oat varie-ty and different fertilizer and legume mixtures,an orthogonality experiment has been conducted in an alpine ar-ea.The experiment consists of three factors (oat varieties,fertilizer and legume mixtures)and four levels made up of (1)four oat varieties:Avena sativa cv.Qingyan No.1,A.sativa cv.Lena,A.sativa cv.Qinghai 444,A.sativa cv.Qinghai;(2)four fertilizer mixes:CK0 ,urea (75 kg/ha)+NH4 H2 PO3 (150 kg/ha),or-ganic manure (1500 kg/ha),urea (37.5 kg/ha)+NH4 H2 PO3 (75 kg/ha)+organic manure (750 kg/ha);and (3)fourVicia sativa sow mixture rates:0 kg/ha,45 kg/ha,60 kg/ha,75 kg/ha.The results showed that ap-propriate combinations of oat variety,fertilizer mixes and legume sow rates can significantly improve soil enzy-matic activities.The activities of soil urease,cellulose and invertase were respectively in the range of 400-900μg/g,80-180 μg/g and 4-7 mg/g in the cultivated alpine soil.Ranked from high to low,the factors that in-fluenced soil enzymatic activities were first fertilizer mix,then variety,then legume mixture rate.Soil urease activity first increased and then decreased during the growth period,peaking at anthesis;cellulose activity first decreased and then increased,peaking at jointing stage;invertase activity had two periods of increase followed by decrease,with double peaks at the jointing and heading stages.Soil enzyme dynamics were calculated using the relative enzymes activity index and the relative enzyme activity comprehensive index.The highest soil enzy-matic activities were recorded in the treatment consisting of the Qinghai 444 or Qinghai sweat oat variety mixed with 45 kg/haV.sativa and fertilized with 37.5 kg/ha urea,75 kg/ha NH4 H2 PO3 and 750 kg/ha organic manure.关键词
高寒区/品种/施肥水平/豆科混播水平/土壤酶活性Key words
alpine area/variety/fertilization rate/legume mixed sowing/soil enzymatic activity引用本文复制引用
刘文辉,张英俊,师尚礼,贺永娟,孙建,魏小星..高寒区施肥和豆科混播水平对燕麦人工草地土壤酶活性的影响[J].草业学报,2017,26(1):23-33,11.基金项目
“现代农业产业技术体系建设专项资金”（CARS-35-41），农业部“青藏高原牧草种质资源保护利用”项目（13162130135252040）和青海省饲草产业科技创新平台项目资助。 （）
