草业学报2017，Vol.26Issue(1)：149-159,11.DOI:10.11686/cyxb2016263
黄芪根际促生菌（PGPR）筛选与特性研究
Identification of plant growth promoting rhizobacteria Astragalus membranaceus and their effectives
摘要
Abstract
In order to obtain and study the performance of plant growth promoting rhizobacteria (PGPR)in As-tragalus membranaceus ,the root nodule,root morphology and rhizosphere soil of A.membranaceus were col-lected.Strains of rhizobium and phosphate solubilizing bacteria were isolated and assessed for the potentially useful characteristics of high nitrogenase activity in the rhizobium and high phosphate solubilisation and ability to secrete 3-indoleacetic acid (IAA)in the phosphate solubilizing bacteria.Potential PGPR strains were then i-dentified using physiological and biochemical characteristics and 16S rDNA sequence analysis.Results showed there are large amounts of phosphorus-dissolving bacteria in the rhizospheres of A.membranaceus .The quan-titative distribution of bacteria and PGPR shows a strong rhizosphere effect,with rhizosplan or surface of roots (RP)>soil adhering to roots (RS)>soil away from roots (NRS)>histoplan or interior of roots (HP).We have isolated 76 PGPR strains,composed of 1 rhizobium,42 inorganic phosphate solubilizing and 33 organic phosphate solubilizing bacteria strains.There are 7 phosphate solubilizing strains with the ability to secrete IAA.A further 8 potential PGPR strains were identified (1 rhizobium and 7 phosphate solubilizing).3 phos-phate solubilizing strains were identified as Pseudomonas sp.,3 phosphate solubilizing strains as Bacillus sp., 1 phosphate solubilizing strain as Klebsiella oxytoca ,1 rhizobium strain as Rhizobium sp.This study has iden-tified potential PGPR for the development of microbial fertilizers for A.membranaceus .关键词
黄芪/根际促生菌(PGPR)/促生特性/菌株鉴定Key words
Astragalus membranaceus/plant growth promoting rhizobacteria (PGPR)/characteristics of the growth/strains identification引用本文复制引用
马骢毓,张英,马文彬,李建宏,姚拓..黄芪根际促生菌（PGPR）筛选与特性研究[J].草业学报,2017,26(1):149-159,11.基金项目
国家自然基金（31360584和31260025）资助。 （）
