口腔疾病防治2017，Vol.25Issue(2)：87-92,6.DOI:10.12016/j.issn.2096-1456.2017.02.004
关节腔内早期注射阿仑膦酸钠对卵巢摘除大鼠髁突软骨下骨的影响
Effect of early intra-articular injection of alendronate in reducing subchondral bone loss in rat mandibular condyle after ovariectomy
摘要
Abstract
[Absract]Objective To investigate the effects of intra⁃articular injection of alendronate on the mandibular condyle in ovariectomized rats. Methods Sixty female rats were randomly divided into five groups:ovariectomy with vehicle treat⁃ment alone, early alendronate treatment at ovariectomy, late alendronate treatment at 4 weeks after ovariectomy, sham⁃operation with vehicle treatment, and the normal control rats. The changes in subchondral bone were evaluated by micro⁃computed tomography (Micro⁃CT), tartrate⁃resistant acid phosphatase (TRAP) staining and real⁃time quantitative poly⁃merase chain reaction (RT⁃PCR). Results Compared with late alendronate treatment, early alendronate treatment im⁃proved microstructural properties of the subchondral bone, with higher bone volume ratio (46.4+2.5 vs 37.5+2.1;P=0.038), increased trabecular thickness (47.3+1.7 vs 34.6+1.4;P=0.029), elevated trabecular number (8.5+0.6 vs 6.2+0.3;P=0.041) and lower trabecular separation (30.2+1.6 vs 37.7+2.6;P=0.034). Fewer TRAP⁃positive cells (4.2+0.2 vs 6.8+0.4;P=0.019) and a higher OPG/RANKL ratio (0.38+0.01 vs 0.25+0.03;P=0.043) in the sub⁃ chondral bone were observed in the animals with early treatment group compared to late treatment or ovariectomy/vehi⁃cle treatment group. Conclusion Our results suggest the therapeutic potential of intra⁃articular alendronate injection in the treatment of osteoporosis⁃associated temporomandibular disorders.关键词
软骨下骨/颞下颌关节/骨质疏松/卵巢切除/给药Key words
Subchondral bone/Temporomandibular joint/Osteoporosis/Ovariectomy/Drug delivery分类
医药卫生引用本文复制引用
李婷,陈瞰,兰玲莉,任笑春,应彬彬,祝颂松..关节腔内早期注射阿仑膦酸钠对卵巢摘除大鼠髁突软骨下骨的影响[J].口腔疾病防治,2017,25(2):87-92,6.基金项目
浙江省自然科学基金（LY12H14002），国家自然科学基金 （）
