摘要
Abstract
Objective To investigate the association of single nucleotide polymorphism of estrogen receptor (GPR30) and cervical cancer. Methods Totally 114 cases of cervical cancer were selected in study group, while the same number of cases of uterine polyps, uterine fibroids and other benign diseases were in control group. GPR30 expression in cervical cancer tissues was determined by S-P immunohistochemical method, and PCR-RFLP method was used for the determination of GPR30 (rs3808351) gene polymorphism. Results There were statistical differences in the times of pregnancies, the first sex age, education level and HPV infection between the study group and the control group (χ2 value was 20. 64, 14. 05. 10. 11 and 39. 71, respectively, all P<0. 05). The positive rate of GPR30 in the study group was significantly higher than that in the control group (χ2 =12. 65, P <0. 05). GG and AA genotypes were significantly different between the study group and the control group (χ2 value was 6. 14 and 5. 89, respectively, both P<0. 05), so were the allele frequencies of G and A (χ2 value was 5. 63 and 5. 76, respectively, both P<0. 05). Logistic regression analysis showed that the number of pregnancies (OR=1. 659, 95% CI:1. 231-4. 964,P<0. 05), HPV infection (OR=1. 778,95% CI:1. 196-3. 854, P<0. 05) and G allele (OR=2. 239, 95% CI:1. 545-7. 032, P<0. 05) were the risk factors for cervical cancer. Conclusion G allele frequency on GPR30 (rs2808350) locus may be the risk factors for cervical cancer.关键词
G蛋白偶联受体30/子宫颈癌/单核苷酸多态性/基因Key words
G-protein coupled receptor 30 (GPR30)/cervical cancer/single nucleotide polymorphism/gene分类
医药卫生
