医学分子生物学杂志2017，Vol.14Issue(1)：10-13,17,5.DOI:10.3870/j.issn.1672-8009.2017.01.003
云南大理猪戊型肝炎病毒分子流行病学调查
Molecular Epidemiology Survey of Swine Hepatitis E Virus in Dali City of Yunnan Province
摘要
Abstract
Objective To investigate the prevalence of swine HEV infection in rural areas of Dali City in Yunnan province, where raw pork is traditionally consumed. Methods HEV ORF2 was detected in swine stool samples ( n =41 ) by reverse transcription⁃nested PCR ( RT⁃nested PCR) . The PCR products were detected by electrophoresis on agarose gel and purified, and then cloned into pMD18⁃T vector and sequenced. The homology and phylogenetic analysis were performed by DNAStar and MEGA4. 0 software, respectively. Results The target gene (348 bp) of HEV was amplified by RT⁃nested PCR. Phylogenetic analysis showed that these isolated viruses were swine HEV genotype 4 and h subtype. In this study, 23 samples were found to have swine HEV, and the positive rate was 56. 10 % ( 23/41 ) . Conclusion Swine HEV infection was highly prevalent in Dali, which indicated a high risk of zoonotic transmission of HEV from swine to humans. Therefore, strategies should be performed to prevent against HEV infection.关键词
猪戊型肝炎病毒/逆转录巢式PCR/进化分析Key words
swine hepatitis E virus/RT-nested PCR/phylogenetic analysis分类
医药卫生引用本文复制引用
彭福春,刘承波,曹文涛,李云龙,龙飞燕,王珏,黄芬..云南大理猪戊型肝炎病毒分子流行病学调查[J].医学分子生物学杂志,2017,14(1):10-13,17,5.基金项目
国家自然科学基金（No．31360619，81660338）云南省自然科学基金（No．2013FB032）This work was supported by grants from the Nation Natural Science Foun-dation of China （No．31360619，81660338） and the Natural Science Foundation of Yunnan Province （）
