中国实用神经疾病杂志2017，Vol.20Issue(3)：26-28,3.
脑缺血后处理对缺血再灌注脑组织细胞间黏附因子-1和髓过氧化物酶的影响
Postconditioning attenuates cerebral ischemia-reperfusion inj ury by inhibiting intercellular adhesion molecule-1 and myeloperoxidase activity
李富强 1王伟 2白宏英3
作者信息
- 1. 南阳医专第一附属医院神经内科 南阳 473000
- 2. 南阳医学高等专科学校基础医学部 南阳 473000
- 3. 郑州大学第二附属医院神经内科 郑州 450014
- 折叠
摘要
Abstract
Objective To investigate the hypothesis that ischemic postconditioning may protect cerebral ischemic tissue. Methods Twenty healthy male Sprague Dawley rats were randomly split into 4 groups:sham group,ischemia-reperfusion group, ischemic postconditioning group,delayed ischemic postconditioning group.Modles of middle cerebral artery occlusion were estab-lished with the improved Longa-Zea method.Anesthetized rats underwent 30 minutes of ischemia and 24 hours of reperfusion.The volume of cerebral infarction size was identified with 2% triphenyl tetrazolium chloride(TTC)staining.Colorimetry was used to analyze the changes of myeloperoxidase activity.Real-time PCR was used to measure the expression ICAM-1 .Results Postcondi-tioning significantly reduced infarct size(P<0.05),and myeloperoxidase activity(P<0.05).Compared with ischemia-reperfusion group,postconditioning significantly inhibited the expression of ICAM-1 .Nevertheless,delayed postconditioning didn’t have such benefit.Conclusion Postconditioning inhibits the expression of ICAM-1 and neutrophil infiltration to provide a potently anti-ische-mic protection.关键词
脑缺血后处理/脑缺血/再灌注损伤/髓过氧化物酶/细胞间黏附因子-1Key words
Ischemic postconditioning/Cerebral ischemic-reperfusion inj ury/Myeloperoxidase/Intercellular adhesion mole-cule-1分类
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李富强,王伟,白宏英..脑缺血后处理对缺血再灌注脑组织细胞间黏附因子-1和髓过氧化物酶的影响[J].中国实用神经疾病杂志,2017,20(3):26-28,3.
