湖南农业大学学报（自然科学版）2017，Vol.43Issue(1)：31-36,6.DOI:10.13331/j.cnki.jhau.2017.01.006
辣椒突变体库的构建及突变群体表型变异分析
Construction of mutant library and research on phenotypic variation of mutant population in the pepper(Capsicum annuumL.)
摘要
Abstract
In this study, the inbred line ‘6421’ was selected and treated with different concentrations of mutagen (ethyl methane sulfonate(EMS)) to study the effect of EMS treatments on seeds germination and some biochemical characteristics of seedlings, to construct mutant library and analyze the phenotypic traits. The results showed that treated with 1.0% EMS, the germination rate and seedling survival rate were 41.01% and 49.32%, respectively, which were close to those withLD50, the seedlings had the highest enzyme activities of SOD (246.45 U/g), POD (33.99 U/g) and CAT (158.10 U/(g·min)), and the MDA content of seedlings was 1.85 times as much as the control. 562 mutant lines were identified and classified into five categories: leaf, stem, fruit, fertility and growth period mutations, which were accounting for 12.73%, 21.56%, 6.92%, 45.33% and 13.46% of the total mutation respectively.关键词
辣椒/甲基磺酸乙酯/半致死剂量/突变体库Key words
Capsicum annuum L./ethyl methane sulfonate(EMS)/half-lethal dose(LD50)/mutant population分类
农业科技引用本文复制引用
周书栋,杨博智,欧立军,刘周斌,吕俊恒,马艳青,邹学校..辣椒突变体库的构建及突变群体表型变异分析[J].湖南农业大学学报（自然科学版）,2017,43(1):31-36,6.基金项目
湖南省自然科学基金项目(2016JJ2076)；农业部产业技术体系项目(CARS-25-A-8) （2016JJ2076）
