华西口腔医学杂志2017，Vol.35Issue(1)：51-56,6.DOI:10.7518/hxkq.2017.01.007
基于软骨细胞外基质的取向支架的制备及评价
Development and characterization of oriented scaffolds derived from cartilage extracellular matrix
摘要
Abstract
Objective This study aimed to prepare oriented scaffolds derived from a cartilage extracellular matrix (CECM) and to investigate their physicochemical property and compatibility with adipose-derived stem cells (ADSCs).Methods A fresh porcine articular cartilage was cut into pieces. Cartilage nanofibers with diameters of 50-500 nm were collected through homogenization and centrifugation. These nanofibers were then decellularized by using Triton X-100 to produce 6% CECM. The oriented scaffolds derived from the nanoscale CECM were fabricated through unidirectional solidification and lyophili-zation. Afterward, these scaffolds were crosslinked. The physical and chemical performances and cell compatibility of CECM-oriented scaffolds were evaluated.Results The cross-sections of the scaffolds contained homogeneous reticular porous struc-tures with nanofibers on the walls of the pores, and the longitudinal sections revealed vertical tubular structures. Hematoxylin-eosin staining revealed that the scaffolds were red without blue. Toluidine blue, safranin O, and Sirius red staining showed positive results. The porosity, water absorption rate, and vertical compressive elastic modulus of the scaffolds were 95.455%± 0.910%, 95.889%±1.071%, and (40.208±5.097) kPa, respectively.Conclusion The components of the oriented scaffolds derived from CECM are similar to those of native cartilage with favorable biocompatibility. The porous structures and sizes of the scaffolds are suitable for the adhesion, prolife-ration, and infiltration of ADSCs. The oriented scaffolds derived from CECM are relatively optimal for cartilage tissue engineering.关键词
软骨/细胞外基质/取向支架/组织工程/定向结晶/冷冻干燥Key words
cartilage/extracellularmatrix/orientedscaffolds/tissueengineering/unidirectionalsolidification/lyophilization分类
生物科学引用本文复制引用
李坤,赵艳红,徐晨,王连永,杨强,李洪发,滕彬宏..基于软骨细胞外基质的取向支架的制备及评价[J].华西口腔医学杂志,2017,35(1):51-56,6.基金项目
国家自然科学基金（31300798，31470937，81572154）；天津市卫生局科技攻关重点项目 （）
