基础医学与临床2017，Vol.37Issue(2)：211-216,6.
干扰ELK-3抑制人肝癌细胞的上皮-间质转换
ELK-3 interference inhibits the epithelial-mesenchymal transition of human hepatocellular carcinoma cells
摘要
Abstract
Objective To investigate the relationship of ELK-3 and epithelial-mesenchymal transition ( EMT) for ex-ploring its possible mechanism .Methods The human hepatocellular carcinoma cells ( HCC) were divided into small interference RNA transfection group and Ras-ELK-3 pathway inhibitor group .The protein level of ELK-3 target gene EGR-1 E-cadherin ,vimentin and p38 in HCC were determined by Western blot analysis .Results The protein level of ELK-3 and its target gene EGR-1 in treated human hepatocellular carcinoma cells significantly decreased as compared with the negative control group (P<0.01).The protein level of E-cadherin was significantly increased (P<0.01), while vimentin and p38 were decreased in HCC cells with ELK-3 interference (P<0.01).Conclusions ELK-3 in-terference can inhibit the epithelial-mesenchymal transition of HCC cells by down-regulating p38.关键词
ELK-3/肝癌细胞/上皮间质转换/p38Key words
ELK-3/hepatocellular carcinoma/epithelial-mesenchymal transition/p38分类
医药卫生引用本文复制引用
李天柱,史铁伟,周静,金光虎,张俊毅,郝丹丹,白春英..干扰ELK-3抑制人肝癌细胞的上皮-间质转换[J].基础医学与临床,2017,37(2):211-216,6.基金项目
内蒙古自然科学基金 （）
