江西农业学报2017，Vol.29Issue(2)：1-8,8.DOI:10.19386/j.cnki.jxnyxb.2017.02.01
烟草基因组中A GO 蛋白的生物信息学分析
Bioinformatics Analysis of Argonau te Proteins in Genomeo f Tobaco
摘要
Abstract
This study summarized the information of tobacco argonaute ( AGO) proteins which were registered in NCBI, and predicted and analyzed their compositions , phylogenetic tree, signal peptides, transmembrane domain, subcellular localization, hydrophobicity , hydrophilicity, and secondary /tertiary protein structure.The results showed that 22 AGO proteins existed in the genome of tobacco, and they could be divided into 8 categories.Like the AGO proteins in Arabidopsis thalia na, the AGO proteins in tobacco had 3 branches in phylogenetic tree;these AGO proteins were hydrophilic proteins without both transmembrane domain and signal peptides, and they possessed two characteristic domains ( PAZ and PIWI) with α-helix spreading in the secondary structure of the protein.In addition, the AGO2/4/7/16/PNH1 of tobacco was positioned on nucleus, AGO10 was positioned on cytoplasm, and AGO5 was positioned on mitochondrion.关键词
生物信息学/烟草/Argonaute ( AGO)/分子结构/功能预测Key words
Bioinformatics/Tobacco/Argonaute protein/Molecular structure/Functional prediction分类
农业科技引用本文复制引用
蔡健宇,武晓云,郭玉双,李翔宇..烟草基因组中A GO 蛋白的生物信息学分析[J].江西农业学报,2017,29(2):1-8,8.基金项目
国家自然科学基金项目“烟草抗马铃薯 Y病毒相关miRNA的筛选与功能分析”（31360431）。 （）
