检验医学与临床2016，Vol.13Issue(z2)：3-6,4.DOI:10.3969/j.issn.1672-9455.2016.26.002
大鼠PAH中六种血清学标志物的检测
Analysis of six kinds of serological markers in rat PAH
摘要
Abstract
Objective to find a appropriate marker for the early diagnosis of pulmonary hypertension in serum .Methods 60 rats were randomly divided into normal control group (n=12) and experimental group (n=48) .The experimental group was injected with MCT to make the pulmonary artery hypertension model of rats while the control group was injected with to make the same a-mount of normal saline .NF-κB、TNF-α、COX-2、PGE2、MMP-1 and iNOS levels of serum were determined by ELISA in all groups . Results the results showed that the model was successful .The concentration of NF-κB、TNF-α、COX-2、PGE2、MMP-1、iNOS in 2 ,4 ,6 weeks had statistical significance compared to the control group (P<0 .05) .And the 2 ,4 ,6 weeks in the the experiment were in the the early stage of PAH development .We found that the specificity of NF-κB ,TNF-α,PGE2 ,iNOS was the most obvious in the the early .Conclusion NF-κB ,TNF-α,PGE2 ,iNOS ,in the rat PAH model can reflect the early pathological changes of PAH ,which has important value for the research of early PAH .关键词
肺动脉高压/早期诊断/血清学标志物Key words
pulmonary arterial hypertension/early diagnosis/serological markers引用本文复制引用
胡童谣,高戈,张照奇,魏丹,石婷,罗沙阳,吴翔,霍治..大鼠PAH中六种血清学标志物的检测[J].检验医学与临床,2016,13(z2):3-6,4.基金项目
湖南省自然科学基金（2015JJ4073）；中南大学硕士生自主创新项目（2016zzts501）；中南大学大学生创新创业项目（CX2015536）；湖南省教育厅科学研究项目（15C0145）；海南省卫生厅普通科研课题（14a20026）。 （）
