检验医学与临床2016，Vol.13Issue(z2)：14-16,3.DOI:10.3969/j.issn.1672-9455.2016.26.006
MALDI-TOF MS 在呼吸道感染丝状真菌鉴定中的应用
The application of MALDI-TOF MS technique for the identification of filamentous fungi from respiratory tract infection
摘要
Abstract
Objective Using MALDI-TOF MS technique and morphological method to identify filamentous fungi isolated from respiratory tract ,and to evaluate the role of mass spectrometry analysis for the identification of filamentous fungi .Methods 139 strains filamentous fungi isolated from clinical respiratory specimens were identified by the mass spectrometer (VITEK MS) of Bi-oMerieux after pretreatment ,and the results were compared with the results of traditional morphological method .Results The cor-rect identification rate of the mass spectrometer to 139 strains filamentous fungi was 82 .7% (115/139);the accuracy of Aspergillus reached 90 .8% (108/119) ,the accuracy of Aspergillus fumigatus and Aspergillus flavus were both 100% (77/77、21/21) ,Aspergil-lus niger was 50 .0% (8/16) ,and other aspergillus was 40 .0% (2/5);the accuracy of penicillium was 58 .3% (7/12) ;the accuracy of mucor ;was 0% (0/3) .The most strains of filamentous fungi causing respiratory tract infection is aspergillus species (89 .2% ) ,in order to Aspergillus fumigatus(55 .4% ) ,Aspergillus flavus(15 .1% ) and aspergillus Niger (11 .5% ) .Conclusion MALDI- TOF MS technology can accurately identify common filamentous fungi quickly ,bring a revolutionary breakthrough for identification of filamentous fungi ,but the database for identification of rare filamentous fungi are still to be improved .关键词
MALDI-TOF MS/呼吸道/丝状真菌/鉴定Key words
MALDI-TOF MS/Respiratory tract/Filamentous fungi/Identification引用本文复制引用
贺靖冬,侯敏,李庆,高强,翟志楠,张连祥..MALDI-TOF MS 在呼吸道感染丝状真菌鉴定中的应用[J].检验医学与临床,2016,13(z2):14-16,3.基金项目
天津市卫生局科技基金（2013KZ076）。 （）
