检验医学与临床2016，Vol.13Issue(z2)：26-28,3.DOI:10.3969/j.issn.1672-9455.2016.26.011
多肽微阵列技术在自身免疫性甲状腺疾病诊断中的初步应用
Peptide Microarray Platform Based on iPDMS for Autoimmune Thyroid Disease
摘要
Abstract
Objective To establish peptide microarray applied to diagnosis of thyroid disease .Methods 76 and 93 peptides (20aa) derived from thyroid stimulating hormone receptor (TSHR) and thyroid peroxidase (TPO) ,respectively ,which were syn-thesized and spotted on our chip in the form of microarray .The chip is based on our self-developed initiator integrated Polydimethyl-siloxane (iPDMS) and applied in patients with thyroid disease .Results Peptide microarray exhibited signal response to serum from the patient with positive anti-TSHR antibodies (TRAb) or anti-TPO antibody (TPOAb) .The TRAb and TPOAb-bound peptide repertoires were constructed ,respectively .Conclusion Peptide microarray platform based on iPDMS could be applied in diagnosis of thyoid disease ,which is expected to achieve the thyroid disease clinical laboratory diagnosis from the single indicators into the era of multiple markers .关键词
肽微阵列/抗促甲状腺素受体抗体/抗甲状腺过氧化物酶抗体/甲状腺疾病Key words
Peptide microarray/Anti-TSH receptor antibodies/Anti-thyroid Peroxidase antibody/Thyroid disease引用本文复制引用
宗毅,刘岑,赵超,赵亚萍..多肽微阵列技术在自身免疫性甲状腺疾病诊断中的初步应用[J].检验医学与临床,2016,13(z2):26-28,3.基金项目
江苏省淮安市科技支撑项目（HAS2013007）。 （）
