检验医学与临床2017，Vol.14Issue(2)：161-163,166,4.DOI:10.3969/j.issn.1672-9455.2017.02.004
不同化学发光法系统测定血清人生长激素水平的现状分析
Analysis of serum human growth hormone levels by different chemiluminescent immunoassay systems
摘要
Abstract
Objective To investigate the comparability of human growth hormone (hGH) levels by different chemiluminescence detection system .Methods Totally 75 venous blood samples (including samples at baseline ,30 ,60 ,90 ,120 mins after the GHTT ) were collected from 15 children with the growth hormone stimulation test .Serum hGH levels were measured by three chemilumi‐nescence systems (CLEIA ,ECLIA and CMIA) ,HGH‐R at each time point was calculated ,and Bland‐Altman method was used to analyze the consistency between the two methods .Results (1) CLEIA‐ECLIA was only basal level ,120 mins after CLEIA‐CMIA stimulation ,hGH level had a good consistency .Between the other two detection systems ,hGH levels were not consistent at any time point .(2)After hGH‐R was introduced ,ECLIA‐CMIA ,30 mins after stimulation ,had a good consistency .The remaining time points ,any two detection systems were not consistent .Conclusion Serum hGH levels are only occasionally consistent at specific de‐tection system ,in the excitation test specific time point .The introduction of hGH‐R does not help to improve the comparability of hGH among the detection systems .关键词
人生长激素/生长激素激发试验/化学发光免疫分析系统Key words
human growth hormone/growth hormone provocation test/chemiluminescent immunoassay systems引用本文复制引用
谭延国,张岩,刘楠,田野,李佩,郑芳芳,聂秋燕,刘晴,王晓宁,古媛..不同化学发光法系统测定血清人生长激素水平的现状分析[J].检验医学与临床,2017,14(2):161-163,166,4.基金项目
卫生部医药卫生科技发展研究中心专项课题资助项目（28-5-5）；首都临床特色应用研究（吴阶平）（Z1411020060000）。 （）
