检验医学与临床2017，Vol.14Issue(4)：455-457,460,4.DOI:10.3969/j.issn.1672-9455.2017.04.001
慢性肾小球肾炎唾液蛋白质组无创伤分子诊断模型的研究
Study on non-invasive molecular diagnostic model of saliva proteomics in patients with chronic glomerulonephritis
摘要
Abstract
Objective To explore the specific biomarkers in saliva of Chronic Glomerulonephritis patients using matrix assisted laser desorption/ionization time‐of‐flight mass spectrometry ,MALDI‐TOF MS(MALDI‐TOF‐MS) technique and to establish diag‐nostic model .Methods The saliva protein fingerprints of 43 Chronic Glomerulonephritis cases and 45 healthy controls were detec‐ted by weak cationic‐exchange magnetic beads(MB‐WCX) and then analyzed by MALDI‐TOF‐MS .Then the specifically expressed differential proteins in two groups were used to establish the diagnostic model combined with bioinformatic methods .Results The saliva protein fingerprint data of the two groups were compared and 116 discrepant protein peaks were found (P < 0 .05) .The 3 peaks of m/z 2 499 .11 Da ,2 159 .09 Da and 3 622 .30 Da were used to build the best decision tree model .The accuracy ,sensitivity and specificity of the diagnostic model were 85 .23% (75/88) ,83 .72% (36/43) and 86 .67% (39/45)respectively .Conclusion Pre‐liminary saliva proteome diagnosis model of chronic glomerulonephritis was set up providing new ways and means for the early diag ‐nosis of chronic glomerulonephritis .关键词
肾小球肾炎/分子诊断模型/唾液/蛋白质组/无创伤诊断Key words
chronic glomerulonephritis/molecular diagnostic model/saliva/proteomics/non-invasive diagnosis引用本文复制引用
曹美群,袁媛,孙珂焕,黄飞娟,陈嫚茵,李仲秋,吴正治..慢性肾小球肾炎唾液蛋白质组无创伤分子诊断模型的研究[J].检验医学与临床,2017,14(4):455-457,460,4.基金项目
国家自然科学基金资助项目（No ．81273665）；国家高技术研究发展计划（863计划）（No ．2014AA020900）。 （）
