山东医药2017，Vol.57Issue(3)：5-8,4.DOI:10.3969/j.issn.1002-266X.2017.03.002
胚胎监测仪延时摄像间隔时间对体外受精胚胎发育的影响
Effect of time-lapse interval of embryo monitoring on human embryonic development after in vitro fertilization
摘要
Abstract
Objective To investigate the effect of time-lapse interval of embryo monitoring on human embryonic devel-opment after in vitro fertilization.Methods Totally 168 fertilized embryos were obtained from 24 couples who received the treatment of in vitro fertilization (IVF)and then were randomly divided into 4 groups:groups A,B,C and D,42 embryos in each group.The embryos in the four groups were continuously monitored for 6 days in a CO2 incubator at 37℃with 6%CO2 .Each group′s embryo development was observed by Primo vision embryo monitor and the time-lapse interval in groups A,B,C and D was 5,10,15 and 20 min,respectively.After the embryo monitoring,we found the corresponding monito-ring photos and analog video for each embryo.We recorded the time of each embryo′s multicellular development and the embryo development at 60 h after in vitro fertilization.The proportion of high quality embryos and blastocysts in each group was calculated,and the first cleavage pattern of embryos in each group was recorded.Results The embryos in the group A needed more time to develop to 5 cells than that of embryos in the group D.The embryos in the groups A and B needed more time to develop to 6 cells,7 cells and 8 cells than that of the embryos in the group D.The embryos in the group A needed more time to develop to 6 cells,7 cells,and 8 cells than that of the embryos in the group C (all P <0.05).The embryo in the group A were still in 6-cell stage at 60 h after IVF,the embryo in the group B had not developed to the stage of 7 cells,the embryo in the group C was in the 7-cell stage and the embryo in the group D was 8-cell embryo.The per-centages of high quality embryos and blastocysts in the groups C and D were higher than that of group A,the proportion of blastocysts in the group C and group D was significantly higher than that of group B (all P <0.05).The proportion of em-bryos which had normal cleavage in the group C and group D was higher than that in group A,and the proportion of normal cleavage in the group D was higher than that in the group B.The proportion of non-axis cleavage and non-diploid cleavage in the group C was lower than that in the group A,the proportion of embryos in the group C with an uneven cleavage was higher than that in the group A,and the proportion of non-axis cleavage embryo in the group D was lower than that in the groups A and B (all P <0.05).Conclusion The time-lapse interval of 5 min and 10 min may have some influence on the embryonic development and the effect of using 15 min and 20 min intervals on embryonic development is smaller than that of 5 min and 10 min.关键词
胚胎监测/延时摄像技术/辅助生殖技术/体外受精/胚胎发育/多细胞胚胎Key words
embryo monitoring/time-lapse camera technology/assisted reproductive technology/in vitro fertilization/embryonic development/multicellular embryo分类
医药卫生引用本文复制引用
柴蓓蓓,于成和,李正伟,许益娟,何培,张若鹏..胚胎监测仪延时摄像间隔时间对体外受精胚胎发育的影响[J].山东医药,2017,57(3):5-8,4.基金项目
大理大学博士科研启动费项目（KYBS201612）；九三学社大理州委立项调研课题。 （）
