山东医药2017，Vol.57Issue(3)：13-15,3.DOI:10.3969/j.issn.1002-266X.2017.03.004
米非司酮对孕激素受体M阳性子宫肌瘤细胞增殖、凋亡的影响
Effects of mifepristone on proliferation and apoptosis of progesterone receptor M positive uterine leiomyoma cells
摘要
Abstract
Objective To observe the effect of mifepristone on proliferation and apoptosis of progesterone receptor M (PR-M)positive uterine leiomyoma cells.Methods The specimen were collected from patients undergoing hysterectomy because of uterine fibroids.After the uterine leiomyoma cells were successful primarily cultured,the PR-M positive cells (positive group)and PR-Mnegative cells (negative group)were screened by Western blotting.Cells in the logarithmic growth phase of two groups were added with 0.1,1 ×10 -6 ,1 ×10 -5 and 1 ×10 -4 mol/L mifepristone,respectively.After 48-hour culture,the inhibition of cell proliferation was observed by MTT,and the apoptosis was observed by flow cytome-try.Results The growth of uterine leiomyoma cells slowed down and began to shrink after treatment of mifepristone for 24 h,and began to break at 48 h.With the increasing concentrations of mifepristone,the cell proliferation inhibition rate and apoptosis rate were increased gradually in the two groups (all P <0.05).When the concentration of mifepristone >1 × 10 -6 mol/L,the proliferation inhibition rate and the apoptosis rate of cells in the positive group was lower than that of the negative group (all P <0.05).Conclusion The proliferation is inhibited and the apoptosis increased in the PR-Mpositive uterine leiomyoma cells after mifepristone intervention,but the degree changed less as compared with PR-Mnegative cells.关键词
米非司酮/孕激素受体M/子宫肌瘤细胞/细胞增殖/细胞凋亡Key words
mifepristone/progesterone receptor M/uterine leiomyoma cells/cell proliferation/apoptosis分类
医药卫生引用本文复制引用
封全灵,熊祯祯,王智霆,张倩雯,刘弘扬,胡兴韶,倪凌佩..米非司酮对孕激素受体M阳性子宫肌瘤细胞增殖、凋亡的影响[J].山东医药,2017,57(3):13-15,3.基金项目
河南省高等学校重点科研项目计划（16A320047）。 （）
