山东医药2017，Vol.57Issue(3)：20-24,5.DOI:10.3969/j.issn.1002-266X.2017.03.006
miR-210的红细胞系相关下游靶基因预测
Prediction of miR-210 erythroid cells-related downstream target genes
摘要
Abstract
Objective To predict the downstream target genes related to miR-210 erythroid cells by bioinformatics. Methods NCBI was used to analyze the conservation of the mature sequence of miR-210 in mammals.The online database of bioinformatics was applied to predict miR-210 downstream target genes.The predicted miR-210 downstream target genes were analyzed by GO classification and enrichment using the BINGO insert in Cytoscape.Screening miR-210 downstream tar-get genes involved in erythroid proliferation,differentiation and apoptosis.The downstream target genes of miR-210 were pre-dicted by DAVID and KEGG database after signal transduction pathway enrichment analysis.Targetscan online tools were used to verify the interaction of target genes with miR-210.Results There was a highly conserved nature of miR-210 mature sequence in many species.The number of the predicted target genes highly related to miR-210 was 416.The molecular func-tions of target genes were enriched in protein binding,transmembrane transport activity of ion,enzyme binding and other mo-lecular functions.The biological process were mainly cell signal transduction,synaptic transmission,etc.,and the composi-tions of the cell components were synaptic vesicles,cytoplasm,organelles and so on.We screened 26 miR-210 downstream target genes which were closely related to erythroid proliferation,differentiation and apoptosis.The signal pathways involved in the regulation were mainly cancer signaling pathway,PI3K-Akt signaling pathway and so on.Smad3,Mapk10,Stat5a, Ywhag,Ctgf and Kitl6 genes were closely related to erythroid proliferation and differentiation,during which,Smad3, Mapk10,Stat5a,Ywhag and Kitl might be directly regulated by mir-210,and Ctgf might be indirectly regulated by miR-210. Conclusions Smad3,Mapk10,Stat5a,Ywhag,Ctgf and Kitl6 are initially screened for the downstream target genes related to miR-210 erythroid cells.These target genes may be related to the erythroid proliferation and differentiation.关键词
微小RNA/miR-210/红细胞系/靶基因/生物信息学Key words
micro RNA/miR-210/erythroid cells/target genes/bioinformatics分类
生物科学引用本文复制引用
施继禹,刘芳,丁谨,曹艳,李文俊..miR-210的红细胞系相关下游靶基因预测[J].山东医药,2017,57(3):20-24,5.基金项目
国家自然科学基金资助项目（81441116）；青海省科技厅（应用）基础研究计划项目（2012-Z-729）；青海大学“123高层次人才培养工程”中青年学术带头人基金；青海大学医学院中青年科研基金团队项目（2014-KT-1）；青海大学医学院大学生科技创新基金项目。 （）
