世界中医药2016，Vol.11Issue(12)：2547-2552,6.DOI:10.3969/j.issn.1673-7202.2016.12.009
基于 UPLC-Q/TOF MS 技术的隔药饼灸合募配穴治疗 UC 大鼠的尿液代谢组学研究
Urine Metabonomic Research of Herbs-partition Moxibustion with Mactching Mu Points in UC Rats Based on UPLC-Q/TOF MS
摘要
Abstract
Objective:To explore the mechanism of herbs-partition moxibustion with the matching mu points in treating UC rats based on metabonomic with UPLC-Q/TOF MS.Methods:Sprague-Dawley rats of 27 cases were randomly divided into normal group,model group,herbs-partition moxibustion with the mactching mu points group.The UC rat model was established by drink-ing DSS aqueous solution.In herbs-partition moxibustion with the matching mu points group,each point moxibustion with 2 moxa cones,once per day,and lasting for 14 days.After moxibustion treatment,all groups urine was analyzed with UPLC-Q/TOF MS. The acquired data were analyzed using principal component analysis (PCA)and partial leastsquares discriminant analysis (PLS-DA).The potential biomarkers and metabolic pathways were analyzed by Metlin(http://metlin.scripps.edu/),KEGG(http://www.kegg.jp),HMDB (http://www.hmdb.ca),MetaboAnalyst 3.0 (http://www.metaboanalyst.ca).Results:Under both positive ion model and negative ion model,the separation trends of 3 groups urine were good.Herbs-partition moxibustion with the matching mu points effectively reduced in 6 different metabolites expression,such as L-cysteine,atraric acid、tocopheronic acid, dibutyl malate、L-tyrosine、L-pipecolic acid,which increased in model group.All 6 different metabolites above were mainly related to amino acid metabolic pathways,such as phenylalanine,tyrosine and tryptophan biosynthesis,cysteine and methionine metabo-lism,tyrosine metabolism,and so on.Conclusion:The mechanism of herbs-partition moxibustion with the matching mu points in treating UC may mainly relate to amino acid metabolism.关键词
灸法/代谢组学/超高效液相色谱-四级杆飞行时间质谱/溃疡性结肠炎Key words
Moxibustion/Metabonomic/UPLC-Q/TOF MS/Ulcerative colitis分类
医药卫生引用本文复制引用
吴璐一,周次利,李璟,顾沐恩,朱毅,刘慧荣,秦文政,黄艳,窦传字,袁凌松,陆颖..基于 UPLC-Q/TOF MS 技术的隔药饼灸合募配穴治疗 UC 大鼠的尿液代谢组学研究[J].世界中医药,2016,11(12):2547-2552,6.基金项目
国家重点基础研究发展计划（“973”计划）项目（编号2009CB522900；2015CB554501）；国家自然科学基金青年项目（编号81303033）；上海市科技启明星计划基金资助项目 （）
