世界中医药2016，Vol.11Issue(12)：2553-2557,5.DOI:10.3969/j.issn.1673-7202.2016.12.010
逆灸“天枢”穴干预大鼠结肠组织基因表达谱的实验研究
Genome-wide Gene Expressions under the Effect of Preventative Moxibustion at ST25 in Rats
摘要
Abstract
Objective:To investigate genome-wide gene expressions of preventative moxibustion at ST25 in healthy rats.Methods:A total of 16 male Sprague-Dawley rats were randomly divided into the normal group (NC)and the normal and herb-partitioned moxibustion group (NM).Starting from day 1,the NMgroup received herb-partitioned moxibustion at the Tianshu points (bilater-al,ST25)for 7 days.On day 8,colon tissues were collected under anesthesia with 2% pentobarbital sodium.The effect of herb-partitioned moxibustion at the ST25 in rats was observed on the general conditions,body weights,and HE staining.Three samples from each group were selected for total RNA extraction.The gene expression profile in the colon tissues was analyzed by the RNA-seq combined with bioinformatics.Results:Compared with the NC group,herb-partitioned moxibustion did not take effect on weight and histopathological observation of the colon in healthy rats.The NMgroup had 866 differentially expressed genes in colon tissues compared to the NC group,of which 604 were up-regulated and 262 were down-regulated.KEGG pathway analysis indica-ted that these genes were involved in multiple pathways,including immunity and protein metabolism.Conclusion:Herb-partitioned moxibustion at ST25 alters genome-wide gene expressions in healthy rats,and affects the biological processes and pathways.The treatment can be used as a healthcare treatment against diseases.关键词
逆灸/结肠组织/基因表达谱/炎症性肠病/免疫Key words
Preventative moxibustion/Colon tissue/Gene expression profiling/Inflammatory bowel disease分类
医药卫生引用本文复制引用
黄艳,王晓梅,李璟,顾沐恩,翁志军,张建斌,施茵,马喆,窦传字,刘慧荣,吴焕淦..逆灸“天枢”穴干预大鼠结肠组织基因表达谱的实验研究[J].世界中医药,2016,11(12):2553-2557,5.基金项目
国家重点基础研究发展计划（”973”计划）项目（编号2009CB522900；2015CB554501）；国家自然科学基金青年项目（编号81503659）；中国博士后科学基金面上项目（编号2015M570380）；上海市针灸机制与穴位功能重点实验室资助（编号14DZ2260500）；上海市重点学科资助项目 （）
