山西医科大学学报2017，Vol.48Issue(1)：1-5,5.DOI:10.13753/j.issn.1007-6611.2017.01.001
非洛地平对氧化型低密度脂蛋白诱导的人脐静脉内皮细胞黏附分子表达的影响
Effects of felodipine on the expression of cell adhesion molecule in human umbilical vein endothelial cells induced by oxidized low density lipoprotein
祁杰 1朱火兰 2薛孟华 3钟妮儿 1梁磊 1孙超峰4
作者信息
- 1. 陕西省人民医院心内二科，西安 710068
- 2. 陕西省人民医院心内一科
- 3. 唐都医院胸外科
- 4. 西安交通大学第一附属医院心内科
- 折叠
摘要
Abstract
Objective To investigate the effect of felodipine on the mRNA and protein expression of intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1(VCAM-1) in human umbilical vein endothelial cells induced by oxidized low density lipoprotein and its mechanism of anti-atherosclerosis. Methods The concentration gradient and time of incubation of oxidized low density lipoprotein(ox-LDL) were screened, and then the human umbilical vein endothelial cells(HUVECs) were co-incubated with ox-LDL and different concentration of felodipine(0. 1,1,10 mol/L) for 24 h. Then the mRNA and protein expression levels of ICAM-1 and VCAM-1 were detected by real time-PCR and Western blot. After HUVECs and U937 cells were co-cultured, the morphological changes were observed under light microscope, and the number of HUVECs adhesion was counted and the adhesion rate was calculat-ed. Results With the increase of ox-LDL concentration, the mRNA and protein expression levels of VCAM-1 and ICAM-1 gradually increased, and peaked at the concentration of 25 mg/L(P<0. 05). Felodipine suppressed the mRNA and protein expression of ICAM-1 and VCAM-1(P<0. 05). Adhesion rate of U937 cells to HUVECs were significantly higher in in 25 mg/L ox-LDL group than in 0 mg/L ox-LDL group(P<0. 01). Felodipine(0. 1 mol/L) significantly inhibited the adhesion of HUVECs and U937(P<0. 01). Conclusion felodipine may inhibit the adhesion of monocyte and endothelial cell through down-regulation of ICAM-1, VCAM-1 expres-sion in endothelial cells and play an anti-inflammatory protective effect.关键词
非洛地平/氧化型低密度脂蛋白/人脐静脉内皮细胞/动脉粥样硬化Key words
felodipine/ox-LDL/atherosclerosis/HUVECs分类
医药卫生引用本文复制引用
祁杰,朱火兰,薛孟华,钟妮儿,梁磊,孙超峰..非洛地平对氧化型低密度脂蛋白诱导的人脐静脉内皮细胞黏附分子表达的影响[J].山西医科大学学报,2017,48(1):1-5,5.
