山西医科大学学报2017，Vol.48Issue(1)：16-21,6.DOI:10.13753/j.issn.1007-6611.2017.01.004
AKAP12基因启动子甲基化在哈萨克族食管鳞癌中研究
Promoter methylation of AKAP12 in Kazakh patients with esophageal squamous cell carcinoma
摘要
Abstract
Objective To explore the promoter methylation of AKAP12 gene in patients with esophageal squamous cell carcinoma( ES-CC) , and its relationship with clinical parameters. Methods AKAP12 methylation status was detected in Eca109 treated by 5-aza-CdR or normal culture, and 18 pairs of Kazakh’ s ESCC tissues by methylation specific PCR( MSP) . The expression of AKAP12 in ESCC cell lines Eca109 and 18 pairs of Kazakh ESCC tissues was detected by real time PCR(RT-PCR). Results The methylation rate of AKAP12 and the normal cells was 0 in 5-aza-CdR-treated Eca109 cells and 5. 6%(1/18) in ESCC tissues. The expression of AKAP12 in 5-aza-CdR group was 0. 99 times as high as control group withβ-actin as the internal reference(P=0. 96), 1. 93 times with GAPDH as the internal reference(P=0. 47). The expression of AKAP12 in the tumor tissues of 18 pairs Kazakh’s ESCC was 0. 86 times as high as that of paracancerous tissues withβ-actin as the internal reference(P=0. 74),2. 87 times with GAPDH as the internal reference(P=0. 11). The expression and the methylation rate of AKAP12 were not correlated with sex, morphologic type, differentiation and metasta-ses(P>0. 05). Conclusion The methylation of AKAP12 is relatively low in esophageal squamous cell carcinoma.关键词
食管鳞癌/DNA甲基化/AKAP12Key words
esophageal squamous cell carcinoma/DNA methylation/AKAP12分类
医药卫生引用本文复制引用
耿月华,杨立民,卢晓梅,刘美莲,孟加榕..AKAP12基因启动子甲基化在哈萨克族食管鳞癌中研究[J].山西医科大学学报,2017,48(1):16-21,6.基金项目
解放军175医院苗圃基金资助项目(14Y005) （14Y005）
