山西医科大学学报2017，Vol.48Issue(1)：22-25,4.DOI:10.13753/j.issn.1007-6611.2017.01.005
急进高原胃动力紊乱大鼠胃电起搏区Cajal间质细胞和缝隙连接蛋白43的表达
Expression of interstitial cell of Cajal and Connexin43 in gastric electrical pacing region of rats after ascending to high altitude
摘要
Abstract
Objective To investigate the gastric pacing interstitial cells of Cajal(interstitial cell of Cajal,ICC)and Connexin43( Cx43) expression in rats after ascending to high altitude, and to explore its mechanism. Methods Thirty SD male rats were divided into three groups(n=10 in each group):low altitude group (Xi’an, 400 m), high altitude(4 300 m) 3 d, 14 d groups. The rats’ gastric electrical activity was measured in different groups. Electron microscope was applied to observe the physiological ultrastructure of ICC cells, and the immunofluorescence staining was used to observe the expression of ICC cells and Cx43 in gastric electrical pacing region. Results The amplitude and frequency of gastric electrical activity of rats were the lowest in high altitude 3 d group ( P<0. 05). The gap junctions and organelles of ICC cells were reduced in high altitude 3 d group, and the apoptotic bodies were observed by electron microscope. The expression of ICC cells and Cx43 was also the lowest in high altitude 3 d group(P<0. 05). Conclusion The damages of ICCs,Cx43 abnormal expression, and the destruction of the gap junction may induce the dysfunctions of intercellular substance exchange and signal transmission,which might partly be responsible for the pathogenesis of gastric motility disorders in high altitude-exposed rats.关键词
高原病/胃动力/胃电起搏区/Cajal间质细胞/Cx43连接蛋白/大鼠Key words
mountain sickness/gastric motility/gastric electrical pacing region/interstitial cell of Cajal/Cx43/rat引用本文复制引用
贺巍,朱琳,范兴爱,杨生岳,刘睿年,魏廷青..急进高原胃动力紊乱大鼠胃电起搏区Cajal间质细胞和缝隙连接蛋白43的表达[J].山西医科大学学报,2017,48(1):22-25,4.基金项目
青海省(应用)基础研究计划资助项目(2013-Z-760) （应用）
