山西医科大学学报2017，Vol.48Issue(1)：26-29,4.DOI:10.13753/j.issn.1007-6611.2017.01.006
ARHI对5-FU诱导胃癌细胞周期阻滞和凋亡的影响
Effects of ARHI on 5-FU-induced gastric cancer cell cycle arrest and apoptosis
摘要
Abstract
Objective To elucidate the effects of ARHI on 5-FU-induced gastric cancer cell cycle arrest and apoptosis. Methods BGC-823 gastric cancer cells were stably transfected with recombinant plasmid vector pIRES2-EGFP or pIRES2-EGFP-ARHI for the ex-periments. After 5-fluorouracil(5-FU) stimulation, flow cytometry was used to detect the changes of cell cycle and apoptosis. Western blot was used to detect the protein expression levels of cyclin D1 ,Bax,Bcl-2 and activated caspase-3. Results Both ARHI and 5-FU induced G1 phase arrest in BGC-823 cells,and the percentage of G1 cells increased to 61. 64%± 4. 56%(P<0. 05) and 65. 71%± 4. 89%(P<0. 01), respectively. The combined effect of ARHI and 5-FU was more significant, and the percentage of G1 cells in-creased to 81. 14%± 5. 63%(P<0. 01). ARHI and 5-FU-induced G1 phase arrest was accompanied by down-regulated protein expres-sion of cyclin D1. ARHI had no effect on BGC-823 cell apoptosis,and 5-FU was able to induce significant apoptosis with an apoptotic rate of 7. 22%± 1. 55%(P<0. 01). The combined effect of ARHI and 5-FU was more significant, and the apoptotic rate increased to 17. 34%± 2. 81%(P<0. 01). 5-FU alone or ARHI and 5-FU-induced BGC-823 cell apoptosis were accompanied by increased Bax/Bcl-2 ratio and activated caspase-3 expression. Conclusion ARHI can enhance 5-FU-induced cell cycle arrest and apoptosis in gas-tric cancer cells.关键词
胃癌/抑癌基因/ARHI/5-氟尿嘧啶/细胞周期/细胞凋亡Key words
gastric cancer/tumor suppressor gene/ARHI/5-Fluorouracil/cell cycle/cell apoptosis分类
医药卫生引用本文复制引用
唐海灵,庄坤,左利平,张欣,杨振威,韩坤..ARHI对5-FU诱导胃癌细胞周期阻滞和凋亡的影响[J].山西医科大学学报,2017,48(1):26-29,4.基金项目
国家自然科学基金资助项目(81502084) （81502084）
