山西医科大学学报2017，Vol.48Issue(1)：30-33,4.DOI:10.13753/j.issn.1007-6611.2017.01.007
低危型HPV-11 E6在小鼠骨髓源树突细胞中的定位表达及其诱发凋亡的研究
Location of Low risk HPV-11E6 in bone marrow-derived dendritic cells and its effect on apoptosis
摘要
Abstract
Objective To investigate the location of GFP-11E6 in bone marrow dendritic cells using green fluorescence expression sys-tem and the apoptosis of GFP-11E6-induced dendritic cells. Methods The plasmid GFP-11E6 was constructed and transfected into dendritic cells from mouse bone marrow. The location and expression level of E6 protein were observed under fluorescent microscope. Flow cytometry was used to detect the apoptosis in GFP-11E6-expressed dendritic cells. Results GFP-11E6 was predominantly ex-pressed in the cytoplasm of dendritic cells after transfection,whereas the GFP control was located in both cytoplasm and nuclei by fluo-rescent microscope. The expression fluorescence intensities of GFP-11E6 and GFP were 73. 22,84. 34 at 12 h after transfection, and they increased to the highest level(98. 25,126. 77) at 24 h after transfection. Then the fluorescence intensities decreased to 87. 46, 103. 56 at 48 h after transfection, and 72. 11,97. 45 at 72 h after transfection. Apoptosis was increased in HPV-11E6-expressed den-dritic cells from 24 h after transfection. Conclusion The low risk HPV11-E6 is mainly located in cytoplasm in dendritic cells and can further induce apoptosis.关键词
低危型HPV-11E6/GFP/树突细胞/凋亡Key words
low risk HPV-11E6/GFP/dendritic cells/apoptosis分类
医药卫生引用本文复制引用
孙丽娜,唐璐,吉兴照,司晨琛,李振军..低危型HPV-11 E6在小鼠骨髓源树突细胞中的定位表达及其诱发凋亡的研究[J].山西医科大学学报,2017,48(1):30-33,4.基金项目
国家自然科学基金资助项目(81201273) （81201273）
国家重点研发计划生物安全监测网络系统集成技术研究(2016YFC1200701) （2016YFC1200701）
