现代检验医学杂志2017，Vol.32Issue(1)：113-116,120,5.DOI:10.3969/j.issn.1671-7414.2017.01.031
氧化酶法和钒酸盐法测定血清胆红素的方法比较
Developed for the Determination of Serum Bilirubin between Oxidase Method and Vanadate Oxidation Method
郝树彬 1孙洪厚 2孙秀会 3刘兴翠 4王志全 2孙爱国5
作者信息
- 1. 山东省医疗器械产品质量检验中心/山东省医疗器械生物学评价重点实验室，济南 250101
- 2. 莘县中日友好医院，山东聊城 252400
- 3. 潍坊泽城生物技术有限公司，山东潍坊 261100
- 4. 山东省恒信检测技术开发中心，济南 250101
- 5. 青州荣军医院，山东潍坊 262500
- 折叠
摘要
Abstract
Objective To study reaction principle of bilirubin vanadate oxidation method and bilirubin oxidase method through comparison of the determination results,and discuss similarities and difference between the two methods .Methods 310 ca-ses were measured and analyzed with each method.Abnormal samples were further investigated.Results Fortotal bilirubin, the regression equation obtained wasY=1.065 1X+1.197 2,the correlation coefficientr=0.997 0.For direct bilirubin of a-dults and children greater than 30 days,the regression equation wasY=0.945 9X+0.599 5 and the correlation coefficient r=0.994 4.For neonatal direct bilirubin,the regression equation wasY=0.410 4X+2.756 3 and the correlation coefficient r=0.883 5.The results from vanadate oxidation method were unacceptable for abnormal neonatal serum measurement after serial dilution.Conclusion The overall conclusions were that for the measurement of total bilirubin,and direct bilirubin for adults and children older than 30 days.The correlation between these two methods is in an acceptable range,for measure-ment of neonatal direct bilirubin,the correlation between thesetwo methods was not acceptable.It is not recommended to measure neonatal direct bilirubin by vanadate oxidation method.关键词
总胆红素/直接胆红素/胆红素氧化酶法/钒酸盐氧化法/新生儿直接胆红素Key words
total bilirubin/direct bilirubin/bilirubin oxidase method/vanadate oxidation method/neonatal direct bilirubin分类
医药卫生引用本文复制引用
郝树彬,孙洪厚,孙秀会,刘兴翠,王志全,孙爱国..氧化酶法和钒酸盐法测定血清胆红素的方法比较[J].现代检验医学杂志,2017,32(1):113-116,120,5.
